典型文献
Traceless enzymatic protein synthesis without ligation sites constraint
文献摘要：
Protein synthesis and semisynthesis offer immense promise for life sciences and have impacted pharmaceutical innovation.The absence of a generally applicable method for traceless peptide conjugation with a flexible choice of junction sites remains a bottleneck for accessing many important synthetic targets,however.Here we introduce the PALME(protein activation and ligation with multiple enzymes)platform designed for sequence-unconstrained synthesis and modification of biomacromolecules.The upstream activating modules accept and process easily accessible synthetic peptides and recombinant proteins,avoiding the challenges associated with preparation and manipulation of activated peptide substrates.Cooperatively,the downstream coupling module provides comprehensive solutions for sequential peptide condensation,cyclization and protein N/C-terminal or internal functionalization.The practical utility of this methodology is demonstrated by synthesizing a series of bioactive targets ranging from pharmaceutical ingredients to synthetically challenging proteins.The modular PALME platform exhibits unprecedentedly broad accessibility for traceless protein synthesis and functionalization,and holds enormous potential to extend the scope of protein chemistry and synthetic biology.
文献关键词：
中图分类号：
作者姓名：
Ruifeng Li;Marcel Schmidt;Tong Zhu;Xinyu Yang;Jing Feng;Yu'e Tian;Yinglu Cui;Timo Nuijens;Bian Wu
作者机构：
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Fresenius Kabi iPSUM,I&D Center EnzyPep B.V.,Geleen 6167 RD,the Netherlands
文献出处：
引用格式：
[1]Ruifeng Li;Marcel Schmidt;Tong Zhu;Xinyu Yang;Jing Feng;Yu'e Tian;Yinglu Cui;Timo Nuijens;Bian Wu-.Traceless enzymatic protein synthesis without ligation sites constraint)[J].国家科学评论（英文版）,2022(05):103-113
A类：
Traceless,semisynthesis,traceless,PALME,Cooperatively
B类：
enzymatic,without,ligation,sites,constraint,Protein,offer,immense,promise,life,sciences,have,impacted,pharmaceutical,innovation,absence,generally,applicable,conjugation,flexible,choice,junction,remains,bottleneck,accessing,many,important,targets,however,Here,introduce,activation,multiple,enzymes,platform,designed,sequence,unconstrained,modification,biomacromolecules,upstream,activating,modules,accept,process,easily,accessible,peptides,recombinant,proteins,avoiding,challenges,associated,preparation,manipulation,activated,substrates,downstream,coupling,provides,comprehensive,solutions,sequential,condensation,cyclization,terminal,internal,functionalization,practical,utility,this,methodology,demonstrated,by,synthesizing,series,bioactive,ranging,from,ingredients,synthetically,challenging,modular,exhibits,unprecedentedly,broad,accessibility,holds,enormous,potential,extend,scope,chemistry,biology
AB值：
0.650958
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
