Avian influenza remains a threat to human wellbeing. Hypochlorite derivatives are commonly used as disinfectants to prevent the spread of the disease. The World Health Organization (WHO) has listed chlorine dioxide (ClO 2) as an A1-level, safe, and efficient disinfectant. In this study, we tested the efficacy of ClO 2, in aqueous solution and gas forms, against avian influenza A (H7N9) virus. The virus suspension was mixed with ClO 2 aqueous solutions of various concentrations and for various time intervals. Aliquots of the mixture were then serially diluted, and the 50% tissue culture infective dose (TCID 50) was measured with a hemagglutination test on MDCK cells. ClO 2 gas produced from generators was introduced in a chamber containing the virus suspension in a Petri dish. The infective activity of the surviving virus was measured by the hemagglutination test. An aqueous solution of ClO 2 at 126 μg/mL for 15 s was effective given that no surviving virus was detected with the hemagglutination test. ClO 2 gas at >5 μL/L sustained for 1 h inactivated the virus effectively, while at 2.5 μL/L for 1 h, it only partially inactivated the virus. ClO 2 as gas or aqueous solution at a certain concentration is effective in inactivating the H7N9 virus, and can be applied for the decontamination and disinfection of environments.

Chlorine dioxide;Influenza virus A;H7N9;Disinfection;Aqueous solution;Gas;ClO 2

Sun Zhiping;Qian Yun;Ogata Norio;Cai Xia;Han Wendong;Xie Youhua;Morino Hirofumi;Sogawa Koushirou;Shibata Takashi;Qu Di

BSL-3 Laboratory of Fudan University, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China;R&D Center, Taiko Pharmaceutical Co., Ltd, Kyoto, Japan;Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China

生物安全与健康 （英文）

[1]Sun Zhiping;Qian Yun;Ogata Norio;Cai Xia;Han Wendong;Xie Youhua;Morino Hirofumi;Sogawa Koushirou;Shibata Takashi;Qu Di-.Effect of chlorine dioxide on avian influenza A (H7N9) virus)[J].生物安全与健康 （英文）,2022(01):53-57

Hypochlorite,ClO ,Aliquots

Effect,chlorine,dioxide,avian,influenza,H7N9,virus,Avian,remains,threat,human,wellbeing,derivatives,are,commonly,used,disinfectants,prevent,spread,disease,World,Health,Organization,WHO,has,listed,A1,level,safe,efficient,this,study,tested,efficacy,aqueous,gas,forms,against,suspension,was,mixed,solutions,various,concentrations,intervals,mixture,were,then,serially,diluted,tissue,culture,infective,dose,TCID ,measured,hemagglutination,MDCK,cells,produced,from,generators,introduced,chamber,containing,Petri,dish,activity,surviving,by,An,given,that,no,detected,sustained,inactivated,effectively,while,partially,certain,inactivating,can,applied,decontamination,disinfection,environments,Chlorine,Influenza,Disinfection,Aqueous,Gas

0.492275