典型文献
A microfluidic cell chip for virus isolation via rapid screening for permissive cells
文献摘要：
Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics.Successful cultivation is the gold standard for identifying a virus,according to the Koch postulates.However,this requires screening for a permissive cell line,which is traditionally time-,reagent-and labor-intensive.Here,a simple and easy-to-operate microfluidic chip,formed by seeding a variety of cell lines and culturing them in parallel,is reported for use in virus cultivation and virus-permissive host-cell screening.The chip was tested by infection with two known viruses,enterovirus 71(EV71)and influenza virus H1N1.Infection with EV71 and H1N1 caused significant cytopathic effects(CPE)in RD and MDCK cells,respectively,demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection.Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput,automatic,and unprecedentedly fast way.
文献关键词：
中图分类号：
作者姓名：
Weide Su;Jingjiang Qiu;Ying Mei;Xian-En Zhang;Yong He;Feng Li
作者机构：
State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China;University of Chinese Academy of Sciences,Beijing,100049,China;School of Mechanics and Safety Engineering,Zhengzhou University,Zhengzhou,450001,China;Current address:Wuhan Institute of Biological Products Co.,Ltd.,Wuhan,430070,China;Faculty of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China;National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing,100101,China;State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou,310027,China
文献出处：
引用格式：
[1]Weide Su;Jingjiang Qiu;Ying Mei;Xian-En Zhang;Yong He;Feng Li-.A microfluidic cell chip for virus isolation via rapid screening for permissive cells)[J].中国病毒学,2022(04):547-557
A类：
postulates
B类：
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AB值：
0.577771
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