Since the anti-inflammatory effect of hydrogen has been widely known,it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions.However,hydrogen is slightly soluble in water,which leads to poor effect of oral hydrogen-rich water therapy.In this study,the nano-bubble hydrogen water(nano-HW)(about 0.7 ppm)was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus(SVCV)-infected zebrafish as model.Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group(control group).The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40％after treatment with nano-bubble hydrogen water,and qRT-PCR results showed that SVCV replication was significantly inhibited.Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water.Futhermore,SVCV infection caused reactive oxygen species(ROS)accumulation was significantly reduced upon nano-HW treatment.The level of proinflammatory cytokines IL-1 β,IL-8,and TNF-α was remarkably reduced in the nano-HW-treated group in vivo and in vitro.Taken together,our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish,which suggests that nano-HW can be applied to antiviral research,and provides a novel therapeutic strategy for virus-caused inflammation related disease.

Chen Li;Yiran Cao;Fukuda Kohei;Haihong Hao;Guiqing Peng;Can Cheng;Jing Ye

State Key Laboratory of Agricultural Microbiology,College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China;Wuhan Britain-China School,Wuhan,430070,China;Shenzhen Nano Buddy Technology Co.,Ltd.,Shenzhen,518000,China;MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products,Huazhong Agricultural University,Wuhan,430070,China;State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,430070,China

中国病毒学

[1]Chen Li;Yiran Cao;Fukuda Kohei;Haihong Hao;Guiqing Peng;Can Cheng;Jing Ye-.Nano-bubble hydrogen water:An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model)[J].中国病毒学,2022(02):277-283

viraemia,Futhermore

Nano,bubble,hydrogen,water,An,effective,therapeutic,agent,against,inflammation,related,disease,caused,by,infection,zebrafish,model,Since,has,been,widely,known,was,supposed,that,could,suppress,tissue,damage,inhibiting,virus,reactions,However,slightly,soluble,which,leads,poor,oral,rich,therapy,In,this,study,nano,HW,about,ppm,prepared,its,investigated,utilizing,spring,carp,SVCV,infected,Three,month,old,were,divided,into,treatment,treated,group,aquaculture,control,results,revealed,cumulative,mortality,reduced,after,qRT,showed,replication,significantly,inhibited,Histopathological,examination,staining,greatly,alleviated,reactive,oxygen,species,ROS,accumulation,upon,level,proinflammatory,cytokines,remarkably,vivo,vitro,Taken,together,our,data,demonstrated,first,response,suggests,applied,antiviral,research,provides,novel,strategy

0.460449