After dengue virus(DENV)infection,antibody-dependent enhancement(ADE)is easy to occur when the neutralizing antibody(NAb)gradually decreases to a sub-neutralizing concentration.In this cohort surveillance,we utilized sera samples collected from dengue fever patients at different convalescent phases in Jinghong City,to investigate the dynamic change rule of DENV-specific antibodies,and to analyze the risk of ADE caused by secondary infection with heterologous serotypes DENVs.For baseline serosurvey,191 four-year and 99 six-year sera samples during convalescence were collected in 2017 and 2019,respectively.The positive rate of DENV-specific immunoglobulin G was 98.4％in 2017,which significantly decreased to 82.8％in 2019.The geo-metric mean titer(GMT)of NAb decreased from 1∶155.35 to 1∶46.66.Among 290 overall samples,73 paired consecutive samples were used for follow-up serosurvey.In four-year sera,the GMTs of NAb against DENV-3 and cross-reactive antibodies against DENV-1,DENV-2 and DENV-4 were 1∶167.70,1∶13.80,1∶18.54 and 1∶45.26,respectively,which decreased to 1∶53.18,1∶10.30,1∶14.60 and 1∶8.17 in six-year sera.In age-stratified analysis,due to the increasing number of ADE positive samples from 2017 to 2019 in 31-40 and 51-60 years groups,the risk of ADE in DENV-4 infection was positively associated with the extension of convalescent phase,and the odd ratio was higher than other groups.With the recovery period lengthened,the risk of secondary infection with DENV-1 and DENV-2 was reduced.Our results offer essential experimental data for risk prediction of severe dengue in hyper-endemic dengue areas,and provide crucial scientific insight for the development of effective dengue vaccines.

Yingshuo Ma;Man Li;Lyu Xie;Na Gao;Dongying Fan;Kaihao Feng;Yao Yao;Yong Zhou;Ziyang Sheng;Hongning Zhou;Hui Chen;Jing An

Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China;Yunnan Institute of Parasitic Diseases,Pu'er,665000,China;People's Hospital of Xishuangbanna Dai Autonomous Prefecture,Xishuangbanna,666100,China;Xishuangbanna Mental Health Center,Xishuangbanna,666100,China;Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research and Yunnan Provincial Collaborative Innovation Center for Public Health and Disease Prevention and Control(YPCICPHDPC),Pu'er,665000,China;Experimental Center for Basic Medical Teaching,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China

中国病毒学

[1]Yingshuo Ma;Man Li;Lyu Xie;Na Gao;Dongying Fan;Kaihao Feng;Yao Yao;Yong Zhou;Ziyang Sheng;Hongning Zhou;Hui Chen;Jing An-.Seroepidemiologic study on convalescent sera from dengue fever patients in Jinghong,Yunnan)[J].中国病毒学,2022(01):19-29

Seroepidemiologic,DENVs,serosurvey,GMTs

study,convalescent,sera,from,dengue,fever,patients,Jinghong,Yunnan,After,virus,infection,antibody,dependent,enhancement,ADE,easy,occur,when,neutralizing,NAb,gradually,decreases,sub,concentration,In,this,cohort,surveillance,utilized,samples,collected,different,phases,City,investigate,dynamic,change,rule,specific,antibodies,analyze,risk,caused,by,secondary,heterologous,serotypes,For,baseline,four,six,during,convalescence,were,respectively,rate,immunoglobulin,was,which,significantly,decreased,geo,metric,mean,titer,Among,overall,paired,consecutive,follow,against,cross,reactive,age,stratified,analysis,due,increasing,number,years,groups,positively,associated,extension,odd,higher,than,other,With,recovery,period,lengthened,reduced,Our,results,offer,essential,experimental,data,prediction,severe,hyper,endemic,areas,provide,crucial,scientific,insight,development,effective,vaccines

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