典型文献
A multiple-target mRNA-LNP vaccine induces protective immunity against experimental multi-serotype DENV in mice
文献摘要：
Dengue virus(DENV)is a mosquito-borne virus with a rapid spread to humans,causing mild to potentially fatal illness in hundreds of millions of people each year.Due to the large number of serotypes of the virus,there re-mains an unmet need to develop protective vaccines for a broad spectrum of the virus.Here,we constructed a modified mRNA vaccine containing envelope domain Ⅲ(E-DⅢ)and non-structural protein 1(NS1)coated with lipid nanoparticles.This multi-target vaccine induced a robust antiviral immune response and increased neutralizing antibody titers that blocked all four types of DENV infection in vitro without significant antibody-dependent enhancement(ADE).In addition,there was more bias for Th1 than Th2 in the exact E-DⅢ and NS1-specific T cell responses after a single injection.Importantly,intramuscular immunization limited DENV transmission in vivo and eliminated vascular leakage.Our findings highlight that chimeric allogeneic structural and non-structural proteins can be effective targets for DENV vaccine and that they can prevent the further development of congenital DENV syndrome.
文献关键词：
中图分类号：
作者姓名：
Lihong He;Wenqiang Sun;Limin Yang;Wenjun Liu;Jing Li
作者机构：
CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Institute of Infectious Diseases,Shenzhen Bay Laboratory,Shenzhen,Guangdong,518000,China;Institute of Microbiology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Beijing,100101,China
文献出处：
引用格式：
[1]Lihong He;Wenqiang Sun;Limin Yang;Wenjun Liu;Jing Li-.A multiple-target mRNA-LNP vaccine induces protective immunity against experimental multi-serotype DENV in mice)[J].中国病毒学,2022(05):746-757
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B类：
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AB值：
0.679397
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