典型文献
The miR166 mediated regulatory module controls plant height by regulating gibberellic acid biosynthesis and catabolism in soybean
文献摘要：
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play critical roles in regulating plant growth and development. Here, we used Short Tandem Target Mimic (STTM) technology to generate soybean (Glycine max (L.) Merr.) miRNA knockdown lines and identify miRNAs that regulate plant height, a key agronomic trait that affects yield. STTM166 successfully silenced miR166 in soybean and upregulated the expression of miR166 target genes, such as ATHB14-LIKE. The miR166 knock-down lines (GmSTTM166) displayed a reduced plant height phenotype. Moreover, GmSTTM166 plants contained lower levels of bioactive gibber-ellic acid (GA3) than wild-type plants, and applica-tion of exogenous GA partially rescued the dwarf phenotype of GmSTTM166. Knockdown of miR166 altered the expression of genes involved in GA biosynthesis and catabolism. Further analysis revealed that ATHB14-LIKE directly represses transcription of the GA biosynthesis genes GmGA1 and GmGA2, while activating transcription of the GA catabolic gene GIBBERLLIN 2 OXIDASE 2 (GmGA2ox2). Collectively, these results reveal a pivotal role for miR166 in the genetic control of plant height in soybean, thereby providing in-valuable insights for molecular breeding to improve soybean yield.
文献关键词：
中图分类号：
作者姓名：
Chen Zhao;Jingjing Ma;Yaohua Zhang;Suxin Yang;Xianzhong Feng;Jun Yan
作者机构：
School of Life Sciences,East China Normal University,Shanghai 200241,China;Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,The Chinese Academy of Sciences,Changchun 130102,China
文献出处：
引用格式：
[1]Chen Zhao;Jingjing Ma;Yaohua Zhang;Suxin Yang;Xianzhong Feng;Jun Yan-.The miR166 mediated regulatory module controls plant height by regulating gibberellic acid biosynthesis and catabolism in soybean)[J].植物学报（英文版）,2022(05):995-1006
A类：
STTM166,ATHB14,GmSTTM166,gibber,ellic,GmGA1,GmGA2,GIBBERLLIN,OXIDASE,GmGA2ox2
B类：
miR166,mediated,regulatory,module,controls,height,regulating,gibberellic,acid,biosynthesis,catabolism,soybean,MicroRNAs,miRNAs,are,endogenous,small,coding,that,critical,roles,growth,development,Here,used,Short,Tandem,Target,Mimic,technology,generate,Glycine,max,Merr,knockdown,lines,identify,key,agronomic,trait,affects,yield,successfully,silenced,upregulated,expression,target,genes,such,as,LIKE,displayed,reduced,phenotype,Moreover,plants,contained,lower,levels,bioactive,GA3,than,wild,applica,exogenous,partially,rescued,dwarf,Knockdown,altered,involved,Further,analysis,revealed,directly,represses,transcription,while,activating,catabolic,Collectively,these,results,pivotal,genetic,thereby,providing,valuable,insights,molecular,breeding,improve
AB值：
0.495339
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