Nanoscale metal organic frameworks(NMOFs)have been widely reported in biomedical field for their unique porous structure and tunable multifunctionality.However,when administrated in vivo,the pro-tein corona will be formed on the surface of NMOFs,significantly affecting their biodistribution,pharma-cokinetics and drug release.Few studies paid attention to the protein corona formation process and its influencing factors of NMOFs.As a well-established strategy for altering structure features of NMOFs,the organic ligand modification may have effect on the protein corona formation process,which is to be in-vestigated.In this study,the zirconium(Zr)-based UIO66 was chosen as model NMOFs,the organic ligand of which was modified with amino group(-NH2)or carboxyl group(-COOH)to synthesize UIO66-NH2 and UIO66-2COOH,respectively.Bovine serum albumin(BSA)was chosen as model protein to investigate the protein corona formation process of NMOFs.The current results showed that the-COOH modification remarkably enhanced the BSA adsorption on NMOFs while-NH2 slightly decreased the protein binding affinity.These differences may be ascribed to the two different dominate protein corona formation modes,i.e.,surface coating mode and porous embedded mode.The protein corona formation did not affect the crystal phase of NMOFs but increased the content of a-helix of BSA.Ultimately,upon protein corona for-mation,the cellular uptake of NMOFs was significantly affected.We believe our study will provide a new research paradigm to the design and applications of NMOFs.

School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China;College of Pharmacy,Jinan University,Guangzhou 511443,China

[1]Wenhao Wang;Huihui Liu;Zhengwei Huang;Fangqin Fu;Wenhua Wang;Linjing Wu;Ying Huang;Chuanbin Wu;Xin Pan-.The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks)[J].中国化学快报（英文版）,2022(09):4185-4190

cokinetics,UIO66,2COOH

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