典型文献
Regulation of Migration, Phagocytosis and Apoptosis of Human Neutrophils by Recombinant Human Intestinal Alkaline Phosphatase
文献摘要：
The purpose of this study was to explore the effects of recombinant human intestinal alkaline phosphatase (recIAP) on human neutrophils in vitro, and the migration, phagocytosis, apoptosis in presence and absence of LPS. In this study, freshly extracted human neutrophils were used to establish an inflammatory cell model, and the control group, recIAP group, LPS group and recIAP +LPS group were set up to stimulate the model. The migration of neutrophils was detected by agarose gel drop method. Fluorescent particles and fluorescent probes were added to different treatment groups, and the phagocytic rate of neutrophils and the release of reactive oxygen species (ROS) from neutrophils were detected by flow cytometry. The apoptosis rate of neutrophils was detected by flow cytometry according to Annexin V-FITC apoptosis detection kit. The results showed that regardless of the presence or absence of LPS, recIAP could inhibit the migration of neutrophils, phagocytosis and the release of ROS. In addition, recIAP could weaken the inhibitory effect of LPS on neutrophils apoptosis.
文献关键词：
中图分类号：
作者姓名：
Hui Mi-zhou;Cong Zhen-yu;Jia Xiao-xiao;Guo Tian-tian;Wu Shu-yin;Shuang Bao
作者机构：
School of Life Sciences,Northeast Agricultural University,Harbin 150030,China;School of Animal Technology Sciences,Qingdao Agricultural University,Qingdao 266109,China;School of Food Sciences,Northeast Agricultural University,Harbin 150030,China
文献出处：
引用格式：
[1]Hui Mi-zhou;Cong Zhen-yu;Jia Xiao-xiao;Guo Tian-tian;Wu Shu-yin;Shuang Bao-.Regulation of Migration, Phagocytosis and Apoptosis of Human Neutrophils by Recombinant Human Intestinal Alkaline Phosphatase)[J].东北农业大学学报（英文版）,2022(04):50-61
A类：
Phagocytosis,recIAP
B类：
Regulation,Migration,Apoptosis,Human,Neutrophils,by,Recombinant,Intestinal,Alkaline,Phosphatase,purpose,this,study,was,explore,effects,recombinant,human,intestinal,alkaline,phosphatase,neutrophils,vitro,migration,phagocytosis,apoptosis,presence,absence,freshly,extracted,were,used,establish,inflammatory,cell,model,control,+LPS,set,stimulate,detected,agarose,gel,drop,method,Fluorescent,particles,fluorescent,probes,added,different,treatment,groups,phagocytic,rate,release,reactive,oxygen,species,ROS,from,flow,cytometry,according,Annexin,FITC,detection,kit,results,showed,that,regardless,could,addition,weaken,inhibitory
AB值：
0.498512
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