典型文献
Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes
文献摘要：
The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation, to explore novel peptides and to research the bioactive function. Mouse spleen lymphocytes were isolated and cultured with LPS stimulation (experimental group) or not (control group) to collect intracellular peptides. Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer (Nano LC-Q Exactive Plus) and identified by PEAKS X software. After compared with peptides reported, 131 novel peptides were discovered, which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database. Prediction data showed that they may have dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Finally, two peptides showed better potent inhibition were verified with competitive and noncompetitive modes.
文献关键词：
中图分类号：
作者姓名：
Juan Wang;Yujia Xie;Yuanyuan Luan;Tingting Guo;Shanshan Xiao;Xingxing Zeng;Shaohui Zhang
作者机构：
School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Zhejiang Go Peptides Life Science and Healthcare Technology Co.,Ltd.,Wenzhou 325000,China
文献出处：
引用格式：
[1]Juan Wang;Yujia Xie;Yuanyuan Luan;Tingting Guo;Shanshan Xiao;Xingxing Zeng;Shaohui Zhang-.Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes)[J].食品科学与人类健康（英文）,2022(06):1515-1526
A类：
nanoliter,PEAKS,UMW,noncompetitive
B类：
Identification,dipeptidyl,peptidase,IV,DPP,inhibitory,verification,peptides,from,mouse,lymphocytes,objective,this,study,was,identify,intracellular,bioactive,before,after,lipopolysaccharide,LPS,stimulation,explore,novel,research,function,Mouse,spleen,were,isolated,cultured,experimental,group,not,control,collect,Totally,analyzed,by,liquid,phase,Exactive,quadrupole,ultra,high,resolution,orbitrap,mass,spectrometer,Nano,LC,Plus,identified,software,After,compared,reported,discovered,which,then,predicted,bioactivity,Peptide,Ranker,BIOPEP,database,Prediction,showed,that,they,may,have,Finally,two,better,potent,inhibition,verified,modes
AB值：
0.563151
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