Objective:Due to the complicated compounds and the synergistic effect of multi-compounds,the quality control and assessment of Chinese materia medica(CMM)encounters a great challenge about how to identify the key compounds,which are directly correlated with its efficacy and safety.On the guidance of study on quality marker(Q-Marker),identification of Q-Markers was performed from Hedan Tablet(HDT)by the aid of the"spider-web"mode and hepatotoxicity evaluation derived from our previous researches and literatures.Methods:By the established ultra performance liquid chromatography with photodiode array detector(UPLC-PDA)method,online UPLC-DPPH and offline antioxidant assay,21 candidate compounds of HDT were systematically investigated and comprehensively evaluated by the"spider-web"mode for them properties of Q-Marker based on"content-stability-activity".In addition,the Q-Markers related with hepatotoxicity based on our previous researches and literatures were identified.Results:Salvianolic acid B(SaB),quercetin-3-O-glucuronide(Qug),isoquercitrin(IQ)and hyperoside(Hyp)were adopted as the preferable Q-Markers of HDT according to the shaded area(A)of tested com-pounds in"spider-web"mode.Psoralen(Ps),isopsoralen(IP),psoralenoside(PO)and isopsoralenoside(IPO)were also strongly recommended as Q-Markers closely related with safety by considering hepato-toxicity of the accumulated Ps and IP and conversion between glycoside(PO and IPO)and aglycone(Ps and IP).Conclusion:This study provided scientific evidence for quality control and assessment of HDT,and also provided a meaningful reference for application of Q-Markers in CMM.

Danni Wang;Jiamin Ding;Xiafei Feng;Xin Chai;Jing Yang;Chunlei Liu;Yingzi Zeng;Wanhui Zhou;Yuefei Wang

State Key Laboratory of Component-based Chinese Medicine,Tianjin Key Laboratory of TCM Chemistry and Analysis,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Foreign Language College,Donghua University,Shanghai 201620,China;Shandong Wohua Pharmaceutical Technology Co.,Ltd.,Weifang 261000,China

中草药（英文版）

[1]Danni Wang;Jiamin Ding;Xiafei Feng;Xin Chai;Jing Yang;Chunlei Liu;Yingzi Zeng;Wanhui Zhou;Yuefei Wang-.Identification of Q-Markers from Hedan Tablet by employing"spider-web"mode and taking compounds'hepatotoxicity into account)[J].中草药（英文版）,2022(04):612-621

Hedan,SaB,Qug,isoquercitrin,Psoralen,isopsoralen,psoralenoside,isopsoralenoside

Identification,Markers,from,Tablet,by,employing,spider,web,mode,taking,compounds,hepatotoxicity,into,account,Objective,Due,complicated,synergistic,effect,multi,quality,control,assessment,Chinese,materia,medica,CMM,encounters,great,challenge,about,how,identify,key,which,directly,correlated,its,efficacy,safety,On,guidance,study,marker,identification,was,performed,HDT,aid,evaluation,derived,our,previous,researches,literatures,Methods,By,established,ultra,performance,liquid,chromatography,photodiode,array,detector,UPLC,PDA,method,online,DPPH,offline,antioxidant,assay,candidate,were,systematically,investigated,comprehensively,evaluated,them,properties,content,stability,activity,In,addition,identified,Results,Salvianolic,acid,quercetin,glucuronide,IQ,hyperoside,Hyp,adopted,preferable,according,shaded,area,tested,IPO,also,strongly,recommended,closely,considering,accumulated,conversion,between,glycoside,aglycone,Conclusion,This,provided,scientific,evidence,meaningful,reference,application

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