(+)-Catechin(CE)is mainly found in green and black tea and has many biological effects,such as anti-inflammatory,anti-cancer,anti-viral effects,protecting human organs,especially the kidney.This study aims to identify the circRNAs induced by CE in db/db mice and their roles in diabetic nephropathy progression.After the db/db mice were treated with CE,RNA-seq was performed to identify the differentially expressed circRNA and mRNAs.The ceRNA regulatory network was constructed and analyzed using bioinformatics software and public databases(Cytoscape,ClueGO,MiRWalk,STRING,et al.).Our results revealed that 6 differentially expressed circRNAs are most associated with the cholinergic synapse,neurotrophin signaling pathway,and insulin signaling pathway.Among these,circRNA.5549 and circRNA.4712 might regulate Cd36,Cyp26b1,C8a,Cyp2j13,Grem2 genes through ceRNA regulatory mechanism in the presence of CE treatment.The expanded network of proteins interacting with these 5 genes shows that the TGF-β signaling pathway,signaling pathways regulating pluripotency of stem cell,fat digestion and absorption,and PPAR signaling pathway was highly enriched.Overall,circRNA.5549 and circRNA.4712 exhibit a promotive function in CE-treated db/db mice,especially in circRNA.5549/miR-29a-5P/Cd36 regulatory network,and this evidence suggest that their ceRNA regulatory network might be a therapeutic target for DN in humans.

Chao Chen;Dina Zhu;Shuai Zhang;Wensheng Zhang

Zhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology,Advanced Institute of Natural Sciences,Beijing Normal University,Zhuhai 519087,China;Engineering Research Center of Natural Medicine,Ministry of Education,Advanced Insti-tute of Natural Sciences,Beijing Normal University,Zhuhai 519087,China;International Cooperation Laboratory of Molecular Medicine,Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization,Faculty of Geographical Science,Beijing Normal University,Beijing 100875,China

食品科学与人类健康（英文）

[1]Chao Chen;Dina Zhu;Shuai Zhang;Wensheng Zhang-.Identification of circRNA/miRNA/mRNA regulatory net-work involving(+)-catechin ameliorates diabetic nephropathy mice)[J].食品科学与人类健康（英文）,2022(03):660-668

MiRWalk,Cd36,C8a,Cyp2j13,Grem2

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