OBJECTIVE:To identify the main active components and targets of Huashi Xingyu Qingre recipe(化湿行淤清热方,HXQR)and to investigate its mechanism in the treatment of oral lichen planus(OLP).METHODS:The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was searched to identify the active ingredients and corresponding targets of HXQR.Disease genes were obtained from the GeneCards database,and a"drug-disease regulatory network"was constructed using Cytoscape software and PERL programming language.The STRING database was used to build a protein-protein interaction network.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)terms were analyzed using R software with a Bioconducter plugin.Finally,the results and the efficacy of HXQR in treating OLP were validated in a clinical trial that included enzyme-linked immunosorbent assay(ELISA)testing and observations of the post-treatment changes in clinical symptoms.RESULTS:HXQR contained 167 active components and 261 targets,with 391 disease targets.The intersection of these two categories in a Venn diagram revealed 57 drug-disease common targets.A compound-target network was constructed and revealed that the six key pharmaceutical ingredients of HXQR were quercetin,luteolin,wogonin,kaempferol,beta-carotene,and baicalein.The protein-protein interaction network mainly involved core proteins such as ALB,interleukin-6,and AKT1.Drug-disease common targets were enriched in 1628 GO terms and 117 KEGG terms,mainly involving inflammatory responses,viral infections,and tumor-related pathways.ELISA testing indicated that HXQR inhibited the tumor necrosis factor(TNF)signaling pathway by reducing the expression of interleukin-6,matrix metalloproteinase-9,and intercellular adhesion molecule-1.The clinical symptoms of the patients with OLP were significantly improved after 8 weeks of treatment with HXQR.CONCLUSION:HXQR treats OLP by regulating the TNF signaling pathway,resulting in a marked treatment effect with few adverse effects.

DENG Qianlan;LU Yueting;YAN Lijuan;LU Hualin;JIN Ruizhe;XU Yanzhi;SONG Jing;LIU Tiejun

Department of Stomatology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Stomatology,Hebei Children's Hospital,Shijiazhuang 050000,China;Department of Stomatology,Shijiazhuang Third Hospital,Shijiazhuang 050000,China

中医杂志（英文版）

[1]DENG Qianlan;LU Yueting;YAN Lijuan;LU Hualin;JIN Ruizhe;XU Yanzhi;SONG Jing;LIU Tiejun-.Mechanism of Huashi Xingyu Qingre recipe(化湿行淤清热方)in treating oral lichen planus based on network pharmacology and clinical trial verification)[J].中医杂志（英文版）,2022(02):304-313

Huashi,Xingyu,HXQR,Bioconducter

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