Background : Gastrointestinal (GI) injury is one of the most common side effects of radiotherapy. However, there is no ideal therapy method except for symptomatic treatment in the clinic. Xuebijing (XBJ) is a traditional Chinese medicine, used to treat sepsis by injection. In this study, the protective effects of XBJ on radiation- induced intestinal injury (RIII) and its mechanism were explored.Methods : The effect of XBJ on survival of irradiated C57BL/6 mice was monitored. Histological changes including the number of crypts and the length of villi were evalu-ated by H&E. The expression of Lgr5 + intestinal stem cells (ISCs), Ki67 + cells, villin and lysozymes were examined by immunohistochemistry. The expression of cytokines in the intestinal crypt was detected by RT- PCR. DNA damage and apoptosis rates in the small intestine were also evaluated by immunofluorescence. Results : In the present study, XBJ improved the survival rate of the mice after 8.0 and 9.0 Gy total body irradiation (TBI). XBJ attenuated structural damage of the small intestine, maintained regenerative ability and promoted proliferation and differentia-tion of crypt cells, decreased apoptosis rate and reduced DNA damage in the intes-tine. Elevation of IL- 6 and TNF- α was limited, but IL- 1, TNF- β and IL- 10 levels were increased in XBJ- treated group after irradiation. The expression of Bax and p53 were decreased after XBJ treatment. Conclusions : Taken together, XBJ provides a protective effect on RⅢ by inhibiting inflammation and blocking p53- related apoptosis pathway.

Yinping Dong;YuanYang Zhang;Xinyue Wang;Wenxuan Li;Junling Zhang;Lu Lu;Hui Dong;Saijun Fan;Aimin Meng;Deguan Li

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine , Chinese Academy of Medical Science &Peking Union Medical College , Tianjin , China;Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences , Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC) , Beijing , China

动物模型与实验医学（英文）

[1]Yinping Dong;YuanYang Zhang;Xinyue Wang;Wenxuan Li;Junling Zhang;Lu Lu;Hui Dong;Saijun Fan;Aimin Meng;Deguan Li-.The protective effects of Xuebijing injection on intestinal injuries of mice exposed to irradiation)[J].动物模型与实验医学（英文）,2022(06):565-574

RIII,lysozymes

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