Background:Polycystic ovary syndrome (PCOS) is an endocrine disorder that occurs in women of child-bearing age. Moreover, PCOS patients have decreased pregnancy rates and clomiphene citrate resistance. The traditional Chinese medicine formula Kun-Tai-1S (KT1S), consisting of the seahorse species hippocampus, has been reported to elicit therapeutic effects in patients with PCOS. However, given the limited resources and global demand for wild hippocampus, whether KT1S with or without hippocampus can elicit similar therapeutic effects has not been confirmed. Methods:KT1S and Kun-Tai-1A (KT1A, KT1S without dry hippocampus) were used to treat a letrozole-induced rat model of PCOS with an established disease. The serum levels of testosterone, luteinizing hormone, anti-Müllerian hormone, and estradiol were determined, the luteinizing hormone/follicle-stimulating hormone ratio was determined, and the ovarian pathology was evaluated. Results: Similar to the therapeutic effects of cyproterone acetate, both the KT1S and KT1A treatments reduced the body weight and ovarian and uterine indices in the rats with PCOS. The serum levels of testosterone, anti-Müllerian hormone, and luteinizing hormone and the luteinizing hormone/follicle-stimulating hormone ratio were significantly lower in the KT1S and KT1A treatment groups compared to the model group (P＜0.01 and P＜0.05, respectively). Moreover, the histopathological assessment results suggested that both the KT1S and KT1A treatments significantly ameliorated the PCOS pathology in the rats with an established disease, with a reduced number of cystic and atretic follicles and an increased number of corpora lutea being observed in the ovaries. Notably, there was no obvious difference in the disease outcomes between the KT1S- and KT1A-treated groups. Network pharmacology analysis 8-isopentenyl-kaempferol, and luteolin in KT1A may promote estrogen signaling; furthermore, the nitric oxide regulation pathway is also closely involved. Conclusion: KT1A and KT1S treatments both significantly ameliorated the PCOS-related pathology in rats, suggesting that the hippocampus component is dispensable for KT1S-mediated amelioration. Given the limited resources and global demand for wild hippocampus for use in complementary medicines, our findings may help conserve this species. Together, our results suggest that KT1A is a promising approach for treating PCOS.

Leanne Lee Leung;Jing Xie;Ya-Cun Chen;Kar-Ho Lam;Hei Wan;Su-Lan Yu;Tzi-Bun Ng;George Pak-Heng Leung;Jin Yu;Ren-Min Yao;Shu-Jia Sun;Sydney Chi-Wai Tang;Hai-Yong Chen;Jia Zhao;Zhang-Jin Zhang;Calvin Kai-Fai Lee;Kalin Yan-Bo Zhang;Li-Xing Lao;Yun Feng;Xiang Lin;Wei Meng

School of Chinese Medicine,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China;School of Biomedical Sciences,Faculty of Medicine,The Chinese University of Hong Kong,Hong Kong 999077,China;Department of Pharmacology and Pharmacy,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China;Workstation for Training and Research(Hong Kong Branch),Distinguished Professor Yu Jin Gynaecology of Chinese Medicine&Integrative Medicine,Hong Kong 999077,China;Shanghai Taikuntang Chinese Medicine Hospital,Shanghai 200023,China;Department of Medicine,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China;Department of Obstetrics and Gynaecology,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China;Virginia University of Integrative Medicine,Virginia 22031,USA;Department of Ophthalmology,Peking University Third Hospital,Beijing 100191,China;Workstation of Zhu Nansun,National Master of Chinese Medicine,Hong Kong Branch of Zhu's School of Gynaecology of Chinese Medicine from Shanghai,Hong Kong 999077,China

传统医学研究（英文版）

[1]Leanne Lee Leung;Jing Xie;Ya-Cun Chen;Kar-Ho Lam;Hei Wan;Su-Lan Yu;Tzi-Bun Ng;George Pak-Heng Leung;Jin Yu;Ren-Min Yao;Shu-Jia Sun;Sydney Chi-Wai Tang;Hai-Yong Chen;Jia Zhao;Zhang-Jin Zhang;Calvin Kai-Fai Lee;Kalin Yan-Bo Zhang;Li-Xing Lao;Yun Feng;Xiang Lin;Wei Meng-.Pharmacological efficacy of the traditional Chinese medicinal formula Kun-Tai-1A in the treatment of letrozole-induced polycystic ovary syndrome)[J].传统医学研究（英文版）,2022(03):44-54

KT1S,KT1A,cyproterone,atretic

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