典型文献
LC-MS/MS method for the quantitation of serum tocilizumab in rheumatoid arthritis patients using rapid tryptic digestion without IgG purification
文献摘要：
The quantitation of serum tocilizumab using liquid chromatography tandem-mass spectrometry(LC-MS/MS)method has not been widely applied in clinical settings because of its time-consuming and costly sample pretreatments.The present study aimed to develop a validated LC-MS/MS method for detecting serum tocilizumab by utilizing immobilized trypsin without an immunoglobulin G purification step and evaluate its applicability in the treatment of rheumatoid arthritis(RA)patients administered intrave-nously or subcutaneously with tocilizumab.The tocilizumab-derived signature peptide was deciphered using a nano-LC system coupled to a hybrid quadrupole-orbitrap mass spectrometer.The serum tocili-zumab was rapidly digested by immobilized trypsin for 30 min.The chromatographic peak of the signature peptide and that of the internal standard were separated from the serum digests for a total run time of 15 min.The calibration curve of serum tocilizumab concentration was linear with a range of 2-200 μg/mL.The intra-and inter-day accuracy and relative standard deviation(RSD)were 90.7％-109.4％and＜10％,respectively.The serum tocilizumab concentrations in the RA patients receiving intravenous and subcutaneous injections were 5.8-28.9 and 2.4-63.5 pg/mL,respectively.The serum tocilizumab concentrations using the current method positively correlated with those using the enzyme-linked immunosorbent assay,although a systematic error was observed between these methods.In conclu-sion,a validated LC-MS/MS method with minimal sample pretreatments for monitoring serum tocili-zumab concentrations in RA patients was developed.
文献关键词：
中图分类号：
作者姓名：
Takashi Mochizuki;Kaito Shibata;Takafumi Naito;Kumiko Shimoyama;Noriyoshi Ogawa;Masato Maekawa;Junichi Kawakami
作者机构：
Department of Hospital Pharmacy,Hamamatsu University School of Medicine,Hamamatsu,431-3192,Japan;Department of Pharmacy,Shinshu University Hospital,Matsumoto,Nagano,390-8621,Japan;Third Division,Department of Internal Medicine,Hamamatsu University School of Medicine,Hamamatsu,431-3192,Japan;Department of Laboratory Medicine,Hamamatsu University School of Medicine,Hamamatsu,431-3192,Japan
文献出处：
引用格式：
[1]Takashi Mochizuki;Kaito Shibata;Takafumi Naito;Kumiko Shimoyama;Noriyoshi Ogawa;Masato Maekawa;Junichi Kawakami-.LC-MS/MS method for the quantitation of serum tocilizumab in rheumatoid arthritis patients using rapid tryptic digestion without IgG purification)[J].药物分析学报（英文）,2022(06):852-859
A类：
tryptic,tocili,zumab
B类：
LC,quantitation,serum,tocilizumab,rheumatoid,arthritis,patients,using,digestion,without,IgG,purification,liquid,chromatography,tandem,mass,spectrometry,has,not,been,widely,applied,clinical,settings,because,its,consuming,costly,sample,pretreatments,present,study,aimed,validated,detecting,by,utilizing,immobilized,trypsin,immunoglobulin,step,evaluate,applicability,RA,administered,nously,subcutaneously,derived,signature,peptide,was,deciphered,nano,coupled,hybrid,quadrupole,orbitrap,spectrometer,rapidly,digested,chromatographic,peak,that,internal,standard,were,separated,from,digests,total,run,calibration,curve,linear,range,day,accuracy,relative,deviation,RSD,respectively,concentrations,receiving,intravenous,injections,pg,current,positively,correlated,those,enzyme,linked,immunosorbent,assay,although,systematic,error,observed,between,these,methods,In,conclu,sion,minimal,monitoring,developed
AB值：
0.501595
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
