典型文献
CRISPR/Cas9 technology and its application in horticultural crops
文献摘要：
Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9(CRISPR/Cas9)system has recently become one popular technology due to its efficiency,precision,and simplicity compared with other genome editing tools such as Zinc Finger Nucleases(ZFNs)and Transcription Activator Like Effector Nucleases(TALENs).Horticultural crops provide energy and health-keeping nutrients to humankind.Genome-editing technology has become widely adopted in horticultural breeding with the increasing demand for high yield and better-quality horticultural crops.Here,we describe the CRISPR/Cas9 system construction,its optimization,including sgRNA promoter,sgRNA design,Cas9 protein promoter,SpCas9 variants and orthologs,and vector delivery methods.We also summarized the application of this technology in horticultural plants for stress responses enhancement,fruit quality improvement,and cultivation traits modification.This detailed review was compiled to help establish comprehensive understanding of the CRISPR/Cas9 systems and provide a reference for further developing this technology to manipulate horticultural plant traits effectively.
文献关键词：
中图分类号：
作者姓名：
Yang Liu;Chunling Zhang;Xiaofei Wang;Xiuming Li;Chunxiang You
作者机构：
State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an,Shandong 271018,China
文献出处：
引用格式：
[1]Yang Liu;Chunling Zhang;Xiaofei Wang;Xiuming Li;Chunxiang You-.CRISPR/Cas9 technology and its application in horticultural crops)[J].园艺学报（英文版）,2022(04):395-407
A类：
Nucleases,Effector
B类：
CRISPR,technology,application,horticultural,crops,Clustered,Regularly,Interspaced,Short,Palindromic,Repeats,associated,has,recently,become,one,popular,due,efficiency,precision,simplicity,compared,other,genome,editing,tools,such,Zinc,Finger,ZFNs,Transcription,Activator,Like,TALENs,Horticultural,provide,energy,health,keeping,nutrients,humankind,Genome,widely,adopted,breeding,increasing,demand,high,yield,better,quality,Here,we,describe,construction,optimization,including,sgRNA,promoter,design,protein,SpCas9,variants,orthologs,vector,delivery,methods,We,also,summarized,this,plants,stress,responses,enhancement,fruit,improvement,cultivation,traits,modification,This,detailed,review,was,compiled,help,establish,comprehensive,understanding,systems,reference,further,developing,manipulate,effectively
AB值：
0.654901
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