典型文献
Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
文献摘要：
Fruits and vegetables in the Cucurbitaceae family,such as cucumber,melon,watermelon,and squash,contribute greatly to the human diet.The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement.However,most economically important cucurbit plants,including melon and squash,remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation,limiting the effective use of genome editing technology.In this study,we used an"optimal infiltration intensity"strategy to establish an efficient genetic transformation system for melon and squash.We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon,squash,and cucumber.The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops.
文献关键词：
中图分类号：
作者姓名：
Tongxu Xin;Haojie Tian;Yalin Ma;Shenhao Wang;Li Yang;Xutong Li;Mengzhuo Zhang;Chen Chens;Huaisong Wang;Haizhen Li;Jieting Xu;Sanwen Huang;Xueyong Yang
作者机构：
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture,Sino-Dutch Joint Lab of Horticultural Genomics,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518124,China;College of Horticulture,Northwest A&F University,Yangling 712100,China;Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;Beijing Vegetable Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097,China;Wimi Biotechnology(Jiangsu)Co.,Ltd,Changzhou,213000,China
文献出处：
引用格式：
[1]Tongxu Xin;Haojie Tian;Yalin Ma;Shenhao Wang;Li Yang;Xutong Li;Mengzhuo Zhang;Chen Chens;Huaisong Wang;Haizhen Li;Jieting Xu;Sanwen Huang;Xueyong Yang-.Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants)[J].园艺研究（英文）,2022(05):54-67
A类：
squash,ERECTA
B类：
Targeted,creation,new,mutants,compact,architecture,using,CRISPR,Cas9,genome,editing,by,optimized,genetic,transformation,procedure,cucurbit,plants,Fruits,vegetables,Cucurbitaceae,family,such,cucumber,watermelon,contribute,greatly,human,diet,widespread,technologies,has,accelerated,functional,characterization,improvement,However,most,economically,important,including,remain,recalcitrant,standard,Agrobacterium,tumefaciens,mediated,limiting,effective,technology,In,this,study,used,optimal,infiltration,intensity,strategy,establish,efficient,system,We,harnessed,power,method,target,homologs,receptor,kinase,created,alleles,that,resulted,shorter,internodes,presented,here,enables,stable,mutagenesis,provides,solid,foundation,manipulation,crops
AB值：
0.571352
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
