Base editing using CRISPR technologies is an invaluable tool for crop breeding.One of the major base edi-tors,the adenine base editor(ABE),has been successfully used in both model plants and many crops.However,owing to limited editing efficiency,the ABE has been difficult to apply in polyploid crops such as allohexaploid bread wheat that often require simultaneous mutation of multiple alleles for fast breed-ing.We have designed a wheat high-efficiency ABE(WhieABE),using the newly developed high-activity adenosine deaminase TadA8e.In vivo and in vitro analysis demonstrated the improved applicability of TadA8e over the commonly used TadA7.10.Dinitroaniline is a widely used herbicide with high effective-ness and low toxicity to animals.However,wheat cultivars with tolerance to dinitroaniline are rare,lim-iting the application of dinitroaniline in wheat planting.Using A-to-G editing with WhieABE,we found that a Met-to-Thr mutation in wheat tubulin alleles located on chromosomes 1A,1B,1D,4A,and 4D increased the resistance of wheat to dinitroaniline,revealing a dosage effect of edited tubulins in resis-tance.The WhieABE promises to be a valuable editing tool for accelerating crop improvement and devel-oping herbicide-resistant wheat germplasm.

Huanan Han;Ziwen Wu;Ling Zheng;Jingyi Han;Yi Zhang;Jihu Li;Shujuan Zhang;Genying Li;Changle Ma;Pingping Wang

College of Life Sciences,Shandong Normal University,Wenhua East Road 88,Jinan 250014,Shandong,China;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China

作物学报（英文版）

[1]Huanan Han;Ziwen Wu;Ling Zheng;Jingyi Han;Yi Zhang;Jihu Li;Shujuan Zhang;Genying Li;Changle Ma;Pingping Wang-.Generation of a high-efficiency adenine base editor with TadA8e for developing wheat dinitroaniline-resistant germplasm)[J].作物学报（英文版）,2022(02):368-374

TadA8e,dinitroaniline,WhieABE,TadA7,Dinitroaniline,tubulins

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