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典型文献
CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley
文献摘要:
Nitrogen is a major determinant of grain yield and quality. As excessive use of nitrogen fertilizer leads to environmental pollution and high production costs, improving nitrogen use efficiency (NUE) is funda-mental for a sustainable agriculture. Here, we dissected the role of the barley abnormal cytokinin response1 repressor 1 (HvARE1) gene, a candidate for involvement in NUE previously identified in a genome-wide association study, through natural variation analysis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated gene editing. HvARE1 was predominantly expressed in leaves and shoots, with very low expression in roots under low nitrogen conditions. Agrobacterium-mediated ge-netic transformation of immature embryos (cv. Golden Promise) with single guide RNAs targeting HvARE1 generated 22 T0 plants, from which four T1 lines harbored missense and/or frameshift mutations based on genotyping. Mutant are1 lines exhibited an increase in plant height, tiller number, grain protein content, and yield. Moreover, we observed a 1.5-to 2.8-fold increase in total chlorophyll content in the flag leaf at the grain filling stage. Delayed sen-escence by 10–14 d was also observed in mutant lines. Barley are1 mutants had high nitrogen content in shoots under low nitrogen conditions. These findings demonstrate the potential of ARE1 in NUE improvement in barley.
文献关键词:
作者姓名:
Sakura D.Karunarathne;Yong Han;Xiao-Qi Zhang;Chengdao Li
作者机构:
Western Crop Genetics Alliance,College of Science,Health,Engineering and Education,Murdoch University,Perth,WA 6150,Australia;Western Australian State Agricultural Biotechnology Centre,Murdoch University,Perth,WA 6150,Australia;Department of Primary Industries and Regional Development,Perth,WA 6151,Australia
引用格式:
[1]Sakura D.Karunarathne;Yong Han;Xiao-Qi Zhang;Chengdao Li-.CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley)[J].植物学报(英文版),2022(03):756-770
A类:
HvARE1,response1,are1,ARE1
B类:
CRISPR,Cas9,editing,natural,variation,analysis,demonstrate,potential,improvement,nitrogen,use,efficiency,barley,Nitrogen,major,determinant,grain,yield,quality,excessive,fertilizer,leads,environmental,pollution,high,production,costs,improving,NUE,funda,sustainable,agriculture,Here,we,dissected,role,abnormal,cytokinin,repressor,candidate,involvement,previously,identified,genome,wide,association,study,through,clustered,regularly,interspaced,short,palindromic,repeats,associated,protein,mediated,was,predominantly,expressed,leaves,shoots,very,low,expression,roots,under,conditions,Agrobacterium,netic,transformation,immature,embryos,cv,Golden,Promise,single,guide,RNAs,targeting,generated,T0,plants,from,which,four,lines,harbored,missense,frameshift,mutations,genotyping,Mutant,exhibited,increase,height,tiller,number,content,Moreover,observed,fold,total,chlorophyll,flag,leaf,filling,stage,Delayed,escence,by,also,Barley,mutants,had,These,findings
AB值:
0.592181
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