典型文献
An efficient root transformation system for CRISPR/Cas9-based analyses of shoot-root communication in cucurbit crops
文献摘要：
Cucurbit crops are suitable models for studying long-distance signaling in horticultural plants.Although thousands of substances are graft transmissible in cucurbits,functional studies have been hampered by the lack of efficient genetic transformation systems.Here,we report a convenient and efficient root transformation method for several cucurbit crops that will facilitate studies of functional genes and shoot-root crosstalk.We obtained healthy plants with completely transformed roots and non-transgenic shoots within 6 weeks.Furthermore,we combined this root transformation method with grafting,which allowed for gene manipulation in the rootstock.We validated our system by exploring salt tolerance mechanisms using a cucumber(Cucumis satiuus)/pumpkin(Cucurbita moschata Duch.)(scion/rootstock)graft in which the sodium transporter gene High-affinity K+transporter1(CmoHKT1;1)was edited in the pumpkin rootstock and by overexpressing the pumpkin tonoplast Na+/H+antiporter gene Sodium hydrogen exchanger4(CmoNHX4)in cucumber roots.
文献关键词：
中图分类号：
作者姓名：
Shouyu Geng;Hamza Sohail;Haishun Cao;Jingyu Sun;Zhi Chen;Lijian Zhou;Wenbo Wang;Runwen Ye;Li Yang;Zhilong Bie
作者机构：
Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;Institute of Facility Agriculture,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
文献出处：
引用格式：
[1]Shouyu Geng;Hamza Sohail;Haishun Cao;Jingyu Sun;Zhi Chen;Lijian Zhou;Wenbo Wang;Runwen Ye;Li Yang;Zhilong Bie-.An efficient root transformation system for CRISPR/Cas9-based analyses of shoot-root communication in cucurbit crops)[J].园艺研究（英文）,2022(01):257-264
A类：
cucurbits,rootstock,satiuus,scion,K+transporter1,CmoHKT1,H+antiporter,exchanger4,CmoNHX4
B类：
An,efficient,transformation,CRISPR,Cas9,analyses,communication,crops,are,suitable,models,studying,long,distance,signaling,horticultural,plants,Although,thousands,substances,transmissible,functional,studies,have,been,hampered,by,lack,genetic,systems,Here,report,convenient,method,several,that,will,facilitate,genes,crosstalk,We,obtained,healthy,completely,transformed,transgenic,shoots,within,weeks,Furthermore,combined,this,grafting,which,allowed,manipulation,validated,our,exploring,salt,tolerance,mechanisms,using,cucumber,Cucumis,pumpkin,Cucurbita,moschata,Duch,sodium,High,affinity,was,edited,overexpressing,tonoplast,Na+,Sodium,hydrogen
AB值：
0.513625
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