典型文献
Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis
文献摘要：
Double fertilization is an innovative phenomenon in angiosperms, in which one sperm cell first fuses with the egg cell to produce the embryo, and then the other sperm fuses with the central cell to produce the endosperm. However, the molecular mechanism of the preferential fertilization of egg cells is poorly understood. In this study, we report that two egg cell-secreted aspartic proteases, ECS1 and ECS2, play an important role in pro-moting preferential fertilization of egg cells in Arabidopsis. We show that simultaneous loss of ECS1 and ECS2 function resulted in an approx-imately 20％ reduction in fertility, which can be complemented by the full-length ECS1/2 but not by corresponding active site mutants or by secretion-defective versions of ECS1/2. Detailed phenotypic analysis revealed that the egg cell–sperm cell attachment was compromised in ecs1 ecs2 siliques. Limited pollination assays with cyclin-dependent kinase a1 (cdka;1) pollen showed that preferential egg cell fertilization was impaired in the ecs1 ecs2 mutant. Taken together, these results demonstrate that egg cells secret two aspartic proteases, ECS1 and ECS2, to facil-itate the attachment of sperm cells to egg cells so that preferential fertilization of egg cells is ach-ieved. This study reveals the molecular mecha-nism of preferential fertilization in Arabidopsis thaliana.
文献关键词：
中图分类号：
作者姓名：
Jiahao Jiang;Nils Stührwohldt;Tianxu Liu;Qingpei Huang;Ling Li;Li Zhang;Hongya Gu;Liumin Fan;Sheng Zhong;Andreas Schaller;Li-Jia Qu
作者机构：
State Key Laboratory for Protein and Plant Gene Research,Peking-Tsinghua Center for Life Sciences at College of Life Sciences,Peking University,Beijing 100871,China;Department of Plant Physiology and Biochemistry,Institute of Biology,University of Hohenheim,Stuttgart 70593,Germany
文献出处：
引用格式：
[1]Jiahao Jiang;Nils Stührwohldt;Tianxu Liu;Qingpei Huang;Ling Li;Li Zhang;Hongya Gu;Liumin Fan;Sheng Zhong;Andreas Schaller;Li-Jia Qu-.Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis)[J].植物学报（英文版）,2022(11):2047-2059
A类：
ECS1,ECS2,ecs1,ecs2,cdka,ieved
B类：
Egg,secreted,aspartic,proteases,promote,gamete,attachment,prioritize,fertilization,egg,cells,over,central,Arabidopsis,Double,innovative,phenomenon,angiosperms,which,one,first,fuses,produce,embryo,then,other,endosperm,However,molecular,mechanism,preferential,poorly,understood,In,this,study,report,that,two,play,important,role,moting,We,simultaneous,loss,function,resulted,approx,imately,reduction,fertility,can,be,complemented,by,full,length,but,corresponding,active,site,mutants,secretion,defective,versions,Detailed,phenotypic,analysis,revealed,was,compromised,siliques,Limited,pollination,assays,cyclin,dependent,kinase,a1,pollen,showed,impaired,Taken,together,these,results,demonstrate,facil,itate,so,This,reveals,thaliana
AB值：
0.44104
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