典型文献
PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis
文献摘要：
The organization of the microtubule cytoskeleton is critical for cell and organ morphogenesis. The evolutionarily conserved microtubule-severing enzyme KATANIN plays critical roles in micro-tubule organization in the plant and animal kingdoms. We previously used conical cell of Arabidopsis thaliana petals as a model system to investigate cortical microtubule organization and cell morphogenesis and determined that KATANIN promotes the formation of circum-ferential cortical microtubule arrays in conical cells. Here, we demonstrate that the conserved protein phosphatase PP2A interacts with and dephosphorylates KATANIN to promote the formation of circumferential cortical micro-tubule arrays in conical cells. KATANIN under-goes cycles of phosphorylation and dephos-phorylation. Using co-immunoprecipitation coupled with mass spectrometry, we identified PP2A subunits as KATANIN-interacting pro-teins. Further biochemical studies showed that PP2A interacts with and dephosphorylates KA-TANIN to stabilize its cellular abundance. Sim-ilar to the katanin mutant, mutants for genes encoding PP2A subunits showed disordered cortical microtubule arrays and defective con-ical cell shape. Taken together, these findings identify PP2A as a regulator of conical cell shape and suggest that PP2A mediates KA-TANIN phospho-regulation during plant cell morphogenesis.
文献关键词：
中图分类号：
作者姓名：
Huibo Ren;Jinqiu Rao;Min Tang;Yaxing Li;Xie Dang;Deshu Lin
作者机构：
Basic Forestry and Proteomic Research Center,Fujian Provincial Key Laboratory of Plant Functional Biology,Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Haixia Institute of Sciences and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
文献出处：
引用格式：
[1]Huibo Ren;Jinqiu Rao;Min Tang;Yaxing Li;Xie Dang;Deshu Lin-.PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis)[J].植物学报（英文版）,2022(08):1514-1530
A类：
KATANIN,severing,dephosphorylates,dephos,phorylation,TANIN,katanin
B类：
PP2A,interacts,microtubule,organization,conical,morphogenesis,cytoskeleton,critical,evolutionarily,conserved,enzyme,plays,roles,plant,animal,kingdoms,We,previously,used,Arabidopsis,thaliana,petals,model,system,investigate,cortical,determined,that,promotes,formation,arrays,cells,Here,demonstrate,protein,phosphatase,circumferential,under,goes,cycles,phosphorylation,Using,immunoprecipitation,coupled,mass,spectrometry,identified,subunits,interacting,teins,Further,biochemical,studies,showed,stabilize,cellular,abundance,Sim,ilar,mutants,encoding,disordered,defective,shape,Taken,together,these,findings,identify,regulator,suggest,mediates,regulation,during
AB值：
0.393416
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