The regulation of adult neural stem cells (NSCs) is critical for lifelong neurogenesis. MicroRNAs (miRNAs) are a type of small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signaling networks responsible for several cellular processes. In this study, miR-103-3p was transfected into neural stem cells derived from embryonic hippocampal neural stem cells. The results showed that miR-103-3p suppressed neural stem cell proliferation and differentiation, and promoted apoptosis. In addition, miR-103-3p negatively regulated NudE neurodevelopment protein 1-like 1 (Ndel1) expression by binding to the 3′ untranslated region of Ndel1. Transduction of neural stem cells with a lentiviral vector overexpressing Ndel1 significantly increased cell proliferation and differentiation, decreased neural stem cell apoptosis, and decreased protein expression levels of Wnt3a, β-catenin, phosphor-GSK-3β, LEF1, c-myc, c-Jun, and cyclin D1, all members of the Wnt/β-catenin signaling pathway. These findings suggest that Ndel1 is a novel miR-103-3p target and that miR-103-3p acts by suppressing neural stem cell proliferation and promoting apoptosis and differentiation. This study was approved by the Animal Ethics Committee of Nantong University, China (approval No. 20200826-003) on August 26, 2020.

Wen Li

Department of Human Anatomy,Institute of Neurobiology,Nantong University,Nantong,Jiangsu Province,China;Key Laboratory of Neuroregenerationof Jiangsu and Ministry of Education,Nantong,Jiangsu Province,China;Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China

中国神经再生研究（英文版）

[1]Wen Li-.miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation)[J].中国神经再生研究（英文版）,2022(02):401-408

Ndel1,NudE,Transduction,Nantong

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