Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sen-sitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was mea-sured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the lllumina HiSeq PE1 50 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10 μg/mL LCC sensitized them to paclitaxel,of which the ICso value reduced from 8.644 μmol/L(95％CI:7.315-10.082 μmol/L)to 2.5 μmol/L(95％CI:2.233-2.7882 μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNA-seq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemn-ness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments.

Ke Zhang;Hanyue Ying;Ruping Zhao;Yuanyuan Chen;Qinghua Deng

Center for Radiation Oncology,Affiliated Hangzhou Cancer Hospital,Zhejiang University School of Medicine,Hangzhou 310001,China;The Forth Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou 310053,China

中草药（英文版）

[1]Ke Zhang;Hanyue Ying;Ruping Zhao;Yuanyuan Chen;Qinghua Deng-.Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway)[J].中草药（英文版）,2022(01):111-116

Capilliposide,capillipes,A2780T,capilliposide,bioinformation,stemn

from,Lysimachia,promotes,terminal,differentiations,reverses,paclitaxel,resistance,cells,human,ovarian,cancer,by,regulating,Fos,Jun,pathway,Objective,To,investigate,potential,effect,LCC,stemness,Methods,Cell,Counting,Kit,CCK8,was,used,measure,IC50,values,apoptosis,sured,through,flow,cytometry,Evaluation,markers,performed,immunoblotting,immunostaining,assays,seq,lllumina,HiSeq,PE1,platform,differentially,expressed,genes,DEGs,were,screened,out,analysis,Overexpression,knockdown,achieved,shRNA,transfection,Results,Pre,exposure,sensitized,them,which,ICso,reduced,Exposure,enhanced,induced,inhibited,colony,ALDH1,Myd88,CD44,while,promoting,that,NFATc1,Cathepsin,MMP9,revealed,expressions,FOS,JUN,upregulated,treated,overexpressing,displayed,increased,sensitivity,shared,common,phenotypes,Conclusion,These,findings,suggested,promoted,activating,might,become,novel,therapy,targets,enhances,chemotherapeutic,treatments

0.477212