Genomic deoxyribonucleic acid(DNA)is selected as the ideal carrier for preserving and transmitting the genetic information over the course of evolution.However,the genomic DNA is constantly exposed to var-ious endogenous and environmental threats,causing a diversity of damaged bases,lesions,mismatches and base-pair modifications in the genome,eventually leading to genomic instability and cancers.Base excision repair(BER)is the most important repair mechanism,repairing a variety of DNA damages arising from oxidation,alkylation,methylation,deamination,and hydrolysis reactions.DNA glycosylases are re-sponsible for initiating the first step of the BER pathway through cleaving the N-glycosidic bond between the damaged base and the DNA backbone.However,abnormal DNA glycosylases are associated with a va-riety of diseases such as cancer,cardiovascular disease,neurological disease and inflammation,suggesting the important role of DNA glycosylases in cancer diagnosis and treatment.Therefore,it is highly desirable to monitor the activity of DNA glycosylases,gaining a deep understanding of the restoration process of damaged DNA and clinical diagnosis.Recently,a series of novel DNA glycosylases detection methods with excellent performance have been developed.In this minireview,we summarize the recent advances in DNA glycosylase assays including amplification-free assay and amplification-assisted assay.Firstly,a brief introduction of amplification-free assay for DNA glycosylase is given.Then,amplification-assisted assays for DNA glycosylases are discussed in detail.Ultimately,the conclusion and prospects of the directions of DNA glycosylase assays are provided.

Lili Wang;Huige Zhang;Wei Chen;Hongli Chen;Jianxi Xiao;Xingguo Chen

State Key Laboratory of Applied Organic Chemistry,College of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000,China

中国化学快报（英文版）

[1]Lili Wang;Huige Zhang;Wei Chen;Hongli Chen;Jianxi Xiao;Xingguo Chen-.Recent advances in DNA glycosylase assays)[J].中国化学快报（英文版）,2022(08):3603-3612

glycosylases,sponsible

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