Fresh-seed germination (FSG) impairs peanut production, especially in areas where the peanut harvest season coincides with rainy weather. Developing FSG-resistant cultivars by molecular breeding is expected to mitigate yield loss and quality impairment caused by FSG. However, the genetic control of FSG awaits elucidation. In this study, FSG at 1, 3, 5, 7, and 9 days post-imbibition in three environments were tested, and quantitative-trait loci (QTL) associated with FSG were mapped in a peanut recombinant inbred line population by leveraging existing high-density peanut genetic maps. Of 24 QTL identified in 13 linkage groups, qFSGA04 was a stable major QTL on linkage group 04 (LG04). It was consistently detected in five germination stages and three environments. By designing and validating DNA markers in the confidence interval of qFSGA04, we identified one single-nucleotide polymorphism and one InDel closely associated with FSG that could be used as linked markers for FSG resistance in peanut breeding.

Maoning Zhang;Qing Zeng;Hua Liu;Feiyan Qi;Ziqi Sun;Lijuan Miao;Xiaona Li;Chenyu Li;Debing Liu;Junjia Guo;Mengyuan Zhang;Jing Xu;Lei Shi;Mengdi Tian;Wenzhao Dong;Bingyan Huang;Xinyou Zhang

Innovation Base of Zhengzhou University,Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/State Industrial Innovation Center of Biological Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains,Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement,Zhengzhou 450002,Henan,China;School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450002,Henan,China;State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China;College of Applied Science and Technology of Hainan University,Danzhou 571737,Hainan,Chinae College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China

作物学报（英文版）

[1]Maoning Zhang;Qing Zeng;Hua Liu;Feiyan Qi;Ziqi Sun;Lijuan Miao;Xiaona Li;Chenyu Li;Debing Liu;Junjia Guo;Mengyuan Zhang;Jing Xu;Lei Shi;Mengdi Tian;Wenzhao Dong;Bingyan Huang;Xinyou Zhang-.Identification of a stable major QTL for fresh-seed germination on chromosome Arahy.04 in cultivated peanut (Arachis hypogaea L.))[J].作物学报（英文版）,2022(06):1767-1773

Arahy,qFSGA04,LG04

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