典型文献
Cloning and functional analysis of the promoter of allergen gene Ara h 1 from peanut
文献摘要：
Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites.Seed-specific promoters(SSPs)can direct the expression of genes specifically in seeds to avoid undesirable effects associated with constitutive expression.However,few SSPs have been identified in peanut.Previous studies have shown that some allergen-encoding genes encode seed storage proteins or exhibit seed-specific/preferential expression.In this study,we characterized allergen-encoding genes from across the genomes of Arachis species to explore seed-specific genes.We found that at least 9 out of 16 identified peanut allergen-encoding genes were expressed specifically in the seeds or were preferentially expressed.A 1493-bp promoter fragment of allergen gene Ara h 1(we named it AHSSP6)was isolated from cultivated peanut genome.cis-element analysis showed that three RY repeat elements which usually exsisted in seed or embryo specific promoter sequence were also present in AHSSP6 sequence.Histochemical analysis showed AHSSP6 could drive the expression of a p-glucuronidase(GUS)reporter gene specifically in the seeds or cotyledon tissue of transgenic Arabidopsis,while not in other tissues.These findings indicated that these promoters of allergen genes were candidate SSPs,and AHSSP6 was a novel SSP which could be potentially utilized in peanut improvement.
文献关键词：
中图分类号：
作者姓名：
Cuiling Yuan;Chunjuan Li;Caixia Yan;Xiaobo Zhao;Juan Wang;Yifei Mou;Zhiwei Wang;Quanxi Sun;Shihua Shan
作者机构：
Shandong Peanut Research Institute,Qingdao,China
文献出处：
引用格式：
[1]Cuiling Yuan;Chunjuan Li;Caixia Yan;Xiaobo Zhao;Juan Wang;Yifei Mou;Zhiwei Wang;Quanxi Sun;Shihua Shan-.Cloning and functional analysis of the promoter of allergen gene Ara h 1 from peanut)[J].中国油料作物学报（英文）,2022(01):14-21
A类：
AHSSP6,exsisted,Histochemical
B类：
Cloning,functional,analysis,allergen,from,peanut,Peanut,seeds,are,ideal,bioreactors,production,foreign,recombinant,proteins,secondary,metabolites,Seed,promoters,SSPs,direct,expression,genes,specifically,avoid,undesirable,effects,associated,constitutive,However,few,have,been,identified,Previous,studies,shown,that,some,encoding,encode,storage,exhibit,In,this,study,characterized,across,genomes,Arachis,species,explore,We,found,least,out,were,expressed,preferentially,bp,fragment,named,was,isolated,cultivated,cis,showed,three,RY,repeat,elements,which,usually,embryo,sequence,also,present,could,drive,glucuronidase,GUS,reporter,cotyledon,transgenic,Arabidopsis,while,not,other,tissues,These,findings,indicated,these,candidate,novel,potentially,utilized,improvement
AB值：
0.491196
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