典型文献
High-density genetic mapping identified a major locus for environmental sex expression in pumpkin(Cucurbita moschata Duch.)
文献摘要：
Long-day length and high temperature inhibit sex expression in pumpkin(Cucurbita moschata Duch.),and therefore directly impact the production potential.In this study,female flowering patterns in photoperiod-insensitive(PPIS)and photoperiod-sensitive(PPS)germplasms differed significantly in a moderately long day and high temperature environment.However,both germplasms exhibited a similar response in short day with either low temperature or high temperature environment.Photoperiod sensitivity led to this difference in sex expression be-tween the germplasms.For the traits of 1st female flowering node(FFFN)and number of female flowers(NFF),high-density linkage map construction and quantitative trait locus(QTL)mapping were performed using SLAF-seq technology and 162 F2 individuals generated from PPIS and PPS.In total,4 655 SLAFs were selected and mapped on 20 linkage groups(LGs).The total map length was 2 502.01 cM with an average interval distance of 0.75 cM.Major QTLs for both FFFN and NFF were detected on LG6 with intervals of 7.89 and 17.67 cM and PVE values of 30.5％and 22.9％,respectively.Further analyses of the major locus for FFFN revealed 73 protein-coding genes.Among them,4 were related to sex expression,photoperiod flowering,and hormone response.An InDel(insertion and deletion)marker partially correlated with FFFN of the F2 population was also developed.Our study identified the QTL for the sex expression response to environmental factors using the high-density linkage map.The identified candidate genes and markers will provide useful information about the molecular interaction between the envi-ronment and sex expression and for marker-assisted selection of pumpkin environment-insensitive resources.
文献关键词：
中图分类号：
作者姓名：
Hafiz Muhammad Khalid Abbas;Hexun Huang;Tingquan Wu;Rui Wang;Hu Du;Sen Lu;Shudan Xue;Chunpeng Yao;Qingmin Jin;Yujuan Zhong
作者机构：
Guangdong Key Laboratory for New Technology Research of Vegetables,Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
文献出处：
引用格式：
[1]Hafiz Muhammad Khalid Abbas;Hexun Huang;Tingquan Wu;Rui Wang;Hu Du;Sen Lu;Shudan Xue;Chunpeng Yao;Qingmin Jin;Yujuan Zhong-.High-density genetic mapping identified a major locus for environmental sex expression in pumpkin(Cucurbita moschata Duch.))[J].园艺学报（英文版）,2022(05):593-601
A类：
PPIS,FFFN,SLAFs,LGs,LG6
B类：
High,density,genetic,mapping,identified,major,locus,environmental,sex,expression,pumpkin,Cucurbita,moschata,Duch,Long,day,length,high,temperature,inhibit,therefore,directly,impact,production,potential,this,study,female,flowering,patterns,photoperiod,insensitive,PPS,germplasms,differed,significantly,moderately,long,However,both,exhibited,similar,response,short,either,Photoperiod,sensitivity,difference,For,traits,1st,node,number,flowers,NFF,linkage,construction,quantitative,were,performed,using,seq,technology,F2,individuals,generated,from,total,selected,mapped,groups,was,cM,average,distance,Major,QTLs,detected,intervals,PVE,values,respectively,Further,analyses,revealed,protein,coding,genes,Among,them,hormone,An,InDel,insertion,deletion,partially,correlated,population,also,developed,Our,factors,candidate,markers,will,provide,useful,information,about,molecular,interaction,between,assisted,selection,resources
AB值：
0.459336
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