The spikelet number per spike (SNS) contributes greatly to grain yield in wheat. Identifying various genes that control wheat SNS is vital for yield improvement. This study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci (QTLs) for SNS. One of them (QSns.sau-2SY-2D.1) was reported previously, while the other (QSns.sau-2SY-7A) was newly detected and further analyzed in this study. QSns.sau-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21–40.78％ of the phenotypic variances. QSns.sau-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm 7AL. The contributions and interactions of both major QTLs were further analyzed and discussed. The effect of QSns.sau-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F2:3 population and a panel of 101 high-generation breeding wheat lines. Furthermore, several genes including the previously reported WHEAT ORTHOLOG OF APO1 (WAPO1), an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1 (APO1) related to SNS, were predicted in the interval of QSns.sau-2SY-7A. In summary, these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

DING Pu-yang;MO Zi-qiang;TANG Hua-ping;MU Yang;DENG Mei;JIANG Qian-tao;LIU Ya-xi;CHEN Guang-deng;CHEN Guo-yue;WANG Ji-rui;LI Wei;QI Peng-fei;JIANG Yun-feng;KANG Hou-yang;YAN Gui-jun;WEI Yu-ming;ZHENG You-liang;LAN Xiu-jin;MA Jian

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Ministry of Science and Technology/Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,P.R.China;College of Resources,Sichuan Agricultural University,Chengdu 611130,P.R.China;College of Agronomy,Sichuan Agricultural University,Chengdu 611130,P.R.China;UWA School of Agriculture and Environment and The UWA Institute of Agriculture,Faculty of Science,University of Western Australia,Crawley 6009,Australia

农业科学学报（英文）

[1]DING Pu-yang;MO Zi-qiang;TANG Hua-ping;MU Yang;DENG Mei;JIANG Qian-tao;LIU Ya-xi;CHEN Guang-deng;CHEN Guo-yue;WANG Ji-rui;LI Wei;QI Peng-fei;JIANG Yun-feng;KANG Hou-yang;YAN Gui-jun;WEI Yu-ming;ZHENG You-liang;LAN Xiu-jin;MA Jian-.A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds)[J].农业科学学报（英文）,2022(06):1551-1562

Wheat55K,QSns,2SY,7AL,WHEAT,ORTHOLOG,APO1,WAPO1,ABERRANT,PANICLE,ORGANIZATION

major,stable,wheat,spikelet,number,per,validated,different,genetic,backgrounds,SNS,contributes,greatly,grain,yield,Identifying,various,genes,that,control,vital,improvement,This,study,used,recombinant,inbred,population,genotyped,by,single,nucleotide,polymorphism,array,identify,two,stably,expressed,quantitative,trait,loci,QTLs,One,them,sau,2D,was,reported,previously,while,other,newly,detected,further,analyzed,this,had,high,LOD,value,ranging,from,explained,phenotypic,variances,flanked,markers,AX,cM,interval,chromosome,arm,contributions,interactions,both,were,discussed,effect,successfully,developing,tightly,linked,kompetitive,allele,specific,F2,panel,generation,breeding,lines,Furthermore,several,including,OF,ortholog,rice,related,predicted,In,summary,these,results,revealed,basis,multi,will,facilitate,subsequent,fine,mapping,utilization

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