The foliage of the native grape species Vitis riparia and certain cold-hardy hybrid grapes are particularly susceptible to the insect pest phylloxera,Daktulosphaira vitifoliae Fitch.A previous study using a cold-hardy hybrid grape biparental F1 population(N～125)detected the first quantitative trait locus(QTL)for foliar resistance on chromosome 14,designated as resistance to Daktulosphaira vitifoliae 3(Rdv3).This locus spans a～7-Mbp(10-20 cM)region and is too wide for effective marker-assisted selection or identification of candidate genes.Therefore,we fine mapped the QTL using a larger F1 population,GE1783(N～1023),and genome-wide rhAmpSeq haplotype markers.Through three selective phenotyping experiments replicated in the greenhouse,we screened 184 potential recombinants of GE1783 using a 0 to 7 severity rating scale among other phylloxera severity traits.A 500-kb fine mapped region at 4.8 Mbp on chromosome 14 was identified.The tightly linked rhAmpSeq marker 14_4805 213 and flanking markers can be used for future marker-assisted breeding.This region contains 36 candidate genes with predicted functions in disease resistance(R genes and Bonzai genes)and gall formation(bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase).Disease resistance genes suggest a traditional R-gene-mediated resistance mechanism often accompanied by a hypersensitive response,which has been widely studied in the plant pathology field.A novel resistance mechanism,non-responsiveness to phylloxera gall formation is proposed as a function of the bifunctional dehydratase gene,which plays a role in gallic acid biosynthesis and is important in gall formation.This study has implications for improvement of foliar phylloxera resistance in cold-hardy hybrid germplasm and is a starting place to understand the mechanism of resistance in crops to gall-forming insects.

Lu Yin;Avinash Karn;Lance Cadle-Davidson;Cheng Zou;Jason Londo;Qi Sun;Matthew D.Clark

Department of Horticultural Science,University of Minnesota,Twin Cities,Minnesota 55018,USA;School of Life Science,Arizona State University,Tempe,Arizona 85281,USA;AgReliant Genetics LLC,Lebanon,Indiana 46052,USA;School of Integrative Plant Sciences,Cornell AgriTech,Cornell University,Geneva,New York 14456,USA;Grape Genetics Research Unit,USDA-ARS,Geneva,New York 14456,USA;Institute of Biotechnology,BRC Bioinformatics Facility,Cornell University,Ithaca,New York 14853,USA

园艺研究（英文）

[1]Lu Yin;Avinash Karn;Lance Cadle-Davidson;Cheng Zou;Jason Londo;Qi Sun;Matthew D.Clark-.Candidate resistance genes to foliar phylloxera identified at Rdv3 of hybrid grape)[J].园艺研究（英文）,2022(01):429-440

phylloxera,Rdv3,hardy,Daktulosphaira,vitifoliae,GE1783,rhAmpSeq,recombinants,Bonzai,dehydroquinate

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