Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean. In this study, we utilized two recombinant inbred line populations (RILs, CY, Huachun 2×Wayao; GB, Guizao 1× B13) to identify quantitative trait loci (QTLs) associated with pod shattering in soybean across multiple environments. A total of 14 QTLs for pod shattering were identified in the two RIL populations, which had LOD scores ranging from 2.64 to 44.33 with phenotypic variance explanation (PVE) ranging from 1.33 to 50.85％. One QTL qPS16-1, located on chromosome 16, included a well-known functional gene Pod dehiscence 1 (Pdh1) that was reported previously. Ten new putative QTLs were validated in two RIL populations, and their LOD scores were between 2.55 and 4.24, explaining 1.33 to 2.60％ of the phenotypic variation. Of which four novel QTLs (qPS01-1, qPS03-2, qPS05-1, and qPS07-1) could be detected in two environments where nine genes had specific changes in gene expression. Although the nine genes may have significant effects on pod shattering of soybean, their detailed functions still need to be further explored in the future. The results of this study will facilitate a better understanding of the genetic basis of the pod shattering-resistant trait and benefit soybean molecular breeding for improving pod shattering resistance.

JIA Jia;WANG Huan;CAI Zhan-dong;WEI Ru-qian;HUANG Jing-hua;XIA Qiu-ju;XIAO Xiao-hui;MA Qi-bin;NIAN Hai;CHENG Yan-bo

The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,P.R.China;The Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou 510642,P.R.China;Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,South China Agricultural University,Guangzhou 510642,P.R.China;Beijing Genomics Institute(BGI)Education Center,University of Chinese Academy of Sciences,Shenzhen 518083,P.R.China

农业科学学报（英文）

[1]JIA Jia;WANG Huan;CAI Zhan-dong;WEI Ru-qian;HUANG Jing-hua;XIA Qiu-ju;XIAO Xiao-hui;MA Qi-bin;NIAN Hai;CHENG Yan-bo-.Identification and validation of stable and novel quantitative trait loci for pod shattering in soybean [Glycine max (L.) Merr.])[J].农业科学学报（英文）,2022(11):3169-3184

Huachun,Wayao,Guizao,qPS16,Pdh1,qPS01,qPS03,qPS05,qPS07

Identification,validation,stable,novel,quantitative,trait,loci,pod,shattering,soybean,Glycine,max,Merr,Pod,important,domesticated,which,cause,great,economic,loss,crop,yield,cultivated,In,this,study,utilized,two,recombinant,inbred,line,populations,RILs,CY,B13,identify,QTLs,associated,across,multiple,environments,total,were,identified,had,LOD,scores,ranging,from,phenotypic,variance,explanation,PVE,One,located,chromosome,included,well,known,functional,dehiscence,that,was,reported,previously,Ten,new,putative,validated,their,between,explaining,variation,Of,four,could,detected,where,nine,genes,specific,changes,expression,Although,may,have,significant,effects,detailed,functions,still,need,further,explored,future,results,will,facilitate,better,understanding,genetic,basis,resistant,benefit,molecular,breeding,improving,resistance

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