Powdery mildew(PM),caused by the fungus Microsphaera diffusa,causes severe yield losses in soybean[Glycine max(L)Merr.]under suitable environmental conditions.Identifying resistance genes and devel-oping resistant cultivars may prevent soybean PM damage.In this study,analysis of F1,F2,and F8∶11 recombinant inbred line(RIL)populations derived from the cross between Zhonghuang 24(ZH24)and Huaxia 3(HX3)indicated that adult-plant resistance(APR)to powdery mildew in the soybean cultivar(cv.)ZH24 was controlled by a single dominant locus.A high-density genetic linkage map of the RIL pop-ulation was used for fine mapping.The APR locus in ZH24 was mapped to a 281-kb genomic region on chromosome 16.Using 283 susceptible plants of another F2 population,the candidate region was fine-mapped to a 32.8-kb genomic interval flanked by the markers InDel14 and Gm16_428.The interval har-bored five genes,including four disease resistance(R)-like genes,according to the Williams 82.a2.v1 ref-erence genome.Quantitative real-time PCR assays of candidate genes revealed that the expression levels of Glyma.16g214300 and Glyma.16g214500 were changed by M.diffusa infection and might be involved in disease defense.Rmd_B13 showed all-stage resistance(ASR)to PM in soybean cv.B13.An allelism test in the F2 segregating population from the cross of ZH24 x B13 suggested that the APR locus Rmd_ZH24 and the ASR locus Rmd_B13 may be allelic or tightly linked.These results provide a reference marker-assisted selection in breeding programs.

Qian Zhou;Bingzhi Jiang;Yanbo Cheng;Qibin Ma;Qiuju Xia;Ze Jiang;Zhandong Cai;Hai Nian

The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,Guangdong,China;The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,South China Agricultural University,Guangzhou 510642,Guangdong,China;The Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Provincial Key Laboratory of Crops Genetics and Improvement,Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510642,Guangdong,China;Beijing Genomics Institute(BGI)Education Center,University of Chinese Academy of Sciences,Shenzhen 518083,Guangdong,China

作物学报（英文版）

[1]Qian Zhou;Bingzhi Jiang;Yanbo Cheng;Qibin Ma;Qiuju Xia;Ze Jiang;Zhandong Cai;Hai Nian-.Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24)[J].作物学报（英文版）,2022(04):1103-1110

Zhonghuang,Microsphaera,diffusa,ZH24,Huaxia,InDel14,Gm16,16g214300,16g214500,Rmd,allelism

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