Thermosensitive genic male sterility(TGMS)has been widely used in two-line hybrid rice breeding.Due to hybrid seed production being highly affected by changeable environments,its application scope is limited to some extent.Thus,it is of great importance to identify potential TGMS genes in specific rice varieties.Here,Diannong S-1 xuan(DNS-1X),a reverse TGMS(RTGMS)japonica male sterile line,was identified from Diannong S-1.Genetic analysis showed that male sterility was tightly controlled by a single recessive gene,which was supported by the phenotype of the F1 and F2∶3 populations derived from the cross between DNS-1X and Yunjing 26(YJ26).Combining simple sequence repeat(SSR)markers and bulked segregation analysis(BSA),we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region,which was designated as rtms1-D.It was narrower than the previously reported RTGMS genes rtms1 and tms6(t).The fertility conversion detected in the natural environment showed that DNS-1X was sterile below 28-30℃;otherwise,it was fertile.Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage.This study will provide new re-sources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines.

Xiao Zhang;Guimei Chang;Zihao Wu;Jinpeng Wan;Jun Yang;Feijun Wang;Fang Wang;Diqiu Yu;Peng Xu

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Menglun,Mengla,Yunnan 666303,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;Center of Economic Botany,Core Botanical Gardens,Chinese Academy of Sciences,Menglun,Mengla,Yunnan 666303,China;The Innovative Academy of Seed Design,Chinese Academy of Sciences,Menglun,Mengla,Yunnan 666303,China

植物多样性（英文）

[1]Xiao Zhang;Guimei Chang;Zihao Wu;Jinpeng Wan;Jun Yang;Feijun Wang;Fang Wang;Diqiu Yu;Peng Xu-.Identification and fine mapping of rtms1-D,a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X)[J].植物多样性（英文）,2022(02):213-221

rtms1,thermosensitive,Diannong,Thermosensitive,xuan,RTGMS,Yunjing,YJ26,tms6

Identification,fine,mapping,responsible,reverse,genic,male,sterility,from,1X,has,been,widely,used,two,hybrid,rice,breeding,Due,seed,production,being,highly,affected,by,changeable,environments,its,application,scope,limited,extent,Thus,great,importance,identify,potential,genes,specific,varieties,Here,DNS,japonica,sterile,was,identified,Genetic,analysis,showed,that,tightly,controlled,single,recessive,which,supported,phenotype,F2,populations,derived,cross,between,Combining,simple,sequence,repeat,SSR,markers,bulked,segregation,BSA,kb,region,chromosome,candidate,designated,It,narrower,than,previously,reported,fertility,conversion,detected,natural,below,otherwise,fertile,Histological,further,indicated,pollen,abortion,occurred,young,microspore,stage,This,study,will,provide,new,sources,pave,way,molecular,lines

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