Pod shattering causes severe yield loss in rapeseed(Brassica napus L)under modern agricultural practice.Identification of highly shatter-resistant germplasm is desirable for the development of rapeseed culti-vars for mechanical harvesting.In the present study,an elite line OR88 with strong shatter resistance and a lignified-layer bridge(LLB)structure was identified.The LLB structure was unique to OR88 and co-segregated with high pod-shatter resistance.The LLB structure is differentiated at stage 12 of gynoe-cium development without any gynoecium defects.Genetic analysis showed that LLB is controlled by a single recessive gene.By BSA-Seq and map-based cloning,the resistance gene location was delimited to a 0.688 Mb region on chromosome C09.Transcriptome analysis suggested BnTCP8.C09 as the gene respon-sible for LLB.The expression of BnTCP.C09 was strongly downregulated in OR88,suppressing cell prolif-eration in the pod valve margin.KASP markers linked to the candidate gene were developed.This pod shatter-resistant line could be used in rapeseed breeding programs by direct transfer of the gene with the assistance of the DNA markers.

Wen Chu;Jia Liu;Hongtao Cheng;Chao Li;Li Fu;Wenxiang Wang;Hui Wang;Mengyu Hao;Desheng Mei;Kede Liu;Qiong Hu

Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory for Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,Hubei,China;National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China

作物学报（英文版）

[1]Wen Chu;Jia Liu;Hongtao Cheng;Chao Li;Li Fu;Wenxiang Wang;Hui Wang;Mengyu Hao;Desheng Mei;Kede Liu;Qiong Hu-.A lignified-layer bridge controlled by a single recessive gene is associated with high pod-shatter resistance in Brassica napus L.)[J].作物学报（英文版）,2022(03):638-646

shatter,vars,OR88,LLB,gynoe,gynoecium,BnTCP8,BnTCP

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