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典型文献
Hypocotyl elongation based on HY5 transcription factor in cold resistant winter rapeseed(Brassica napus L.)
文献摘要:
Winter rape(Brassica napus L.)is better than other edible oil crops in China,but poor cold resistance is the key factor restricting its development.Hypocotyl length was found closely related to cold tolerance.The correlation between hypocotyl length and semi-lethal low temperature was significant,and the highest correlation between hypocotyl length and LT50 of autumn sowing was 0.9557.When the hypocotyl were treated at low temperature,the cells were seriously damaged and formed cavity structures,with cell walls seriously damaged or merged into each other.The positive regulation gene of hypocotyl length in resistant line of VHTSG 10 was identified as HY5(transcription factor HY5-like)by qPCR,and bZIP transcription factor was found to be its conserved domain.Fused gene by GFP and HY5 from VHTSG 10 was transient transferred into Nicotiana benthamiana cells.Corresponding to the 35S:GFP widely distributed in plasma membrane of leaf epidermis,the fusion protein 35S:HY5-GFP was mainly distributed in nucleus.Thus we regarded BnHY5 gene is a key gene related to cold tolerance and hypocotyl length in B.napus.
文献关键词:
作者姓名:
Jiaojiao Jin;Wancang Sun;Junyan Wu;Yan Fang;Xuecai Li;Li Ma;Lijun Liu;Rui Zeng
作者机构:
College of Agriculture and Forestry,Longdong University/Gansu Province Dryland Winter Wheat Germplasm Innovation and Application Engineering Research Center/Provincial Collaborative Innovation Center,Qingyang,745000,China;College of Agronomy,Gansu Agricultural University/Gansu Provincial Key Laboratory of Arid Land Crop Sciences/Gansu Research Center of Rapeseed Engineering and Technology/Key Laboratory of Crop Genetics Improvement and Germplasm Enhancement of Gansu Province,Lanzhou,730070,China
引用格式:
[1]Jiaojiao Jin;Wancang Sun;Junyan Wu;Yan Fang;Xuecai Li;Li Ma;Lijun Liu;Rui Zeng-.Hypocotyl elongation based on HY5 transcription factor in cold resistant winter rapeseed(Brassica napus L.))[J].中国油料作物学报(英文),2022(01):40-52
A类:
Hypocotyl,VHTSG,BnHY5
B类:
elongation,transcription,cold,resistant,winter,rapeseed,Brassica,napus,Winter,better,than,other,edible,oil,crops,China,poor,resistance,key,restricting,its,development,length,was,found,closely,related,tolerance,correlation,between,hypocotyl,semi,lethal,low,temperature,significant,highest,LT50,autumn,sowing,When,were,treated,cells,seriously,damaged,formed,cavity,structures,walls,merged,into,each,positive,regulation,gene,line,identified,like,by,qPCR,bZIP,conserved,domain,Fused,GFP,from,transient,transferred,Nicotiana,benthamiana,Corresponding,35S,widely,distributed,plasma,membrane,leaf,epidermis,fusion,protein,mainly,nucleus,Thus,regarded
AB值:
0.49141
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