Artemisia annua is an important preferred host of the mirid bug Apolygus lucorum in autumn. Volatiles emitted from A. annua attract A. lucorum. Volatile artemisinic acid of A. annua is a precursor of artemisinin that has been widely investigated in the Chinese herbal medicine field. However, little is known at this point about the biological roles of artemisinic acid in regulating the behavioral trends of A. lucorum. In this study, we collected volatiles from A. annua at the seedling stage by using headspace solid phase microextraction (HS-SPME). Gas chromatography-mass spectrometry (GC-MS) analysis showed that approximately 11.03±6.00 and 238.25±121.67 ng h–1 artemisinic acid were detected in volatile samples and milled samples, respectively. Subsequently, a key gene for artemisinic acid synthesis, the cytochrome P450 gene cyp71av1, was expressed in engineered Saccharomyces cerevisiae to catalyze the production of artemisinic acid. After the addition of exogenous artemisinic alcohol or artemisinic aldehyde, artemisinic acid was identified as the product of the expressed gene. In electroantennogram (EAG) recordings, 3-day-old adult A. lucorum showed significant electrophysiological responses to artemisinic alcohol, artemisinic aldehyde and artemisinic acid. Furthermore, 3-day-old female bugs were significantly attracted by artemisinic acid and artemisinic alcohol at a concentration of 10 mmol L–1, whereas 3-day-old male bugs were attracted significantly by 10 mmol L–1 artemisinic acid and artemisinic aldehyde. We propose that artemisinic acid and its precursors could be used as potential attractant components for the design of novel integrated pest management strategies to control A. lucorum.

TENG Dong;LIU Dan-feng;Khashaveh ADEL;SUN Pei-yao;GENG Ting;ZHANG Da-wei;ZHANG Yong-jun

State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China;Laboratory of Ecology and Evolutionary Biology,Yunnan University,Kunming 650091,P.R.China;Langfang Scientific Research Trial Station,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Langfang 065000,P.R.China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,P.R.China

农业科学学报（英文）

[1]TENG Dong;LIU Dan-feng;Khashaveh ADEL;SUN Pei-yao;GENG Ting;ZHANG Da-wei;ZHANG Yong-jun-.Biosynthesis of artemisinic acid in engineered Saccharomyces cerevisiae and its attractiveness to the mirid bug Apolygus lucorum)[J].农业科学学报（英文）,2022(10):2984-2994

artemisinic,mirid,Volatiles,cyp71av1

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