典型文献
Suppression of pheromone biosynthesis and mating behavior by RNA interference of pheromone gland-specific fatty acyl reductase in Maruca vitrata
文献摘要：
In moths,various enzymes,such as fatty acid synthases,fatty acyl desaturases,and fatty acyl reductases(FARs),are involved in pheromone biosynthesis.In particular,pheromone gland-specific FAR(pgFAR)plays an important role in converting the func-tional group from carboxylic to alcohol during pheromone biosynthesis.A novel pgFAR of Maruca vitrata,Mvi-pgFAR,was identified through transcriptome sequencing of its pheromone gland.To investigate the involvement of Mvi-pgFAR in pheromone biosynthe-sis,Mvi-pgFAR was cloned from the pheromone gland and suppressed by RNA interfer-ence(RNAi).Mvi-pgFAR harbored several conserved motifs related to NAD(P)H-binding,N-glycosylation,and adenosine/guanosine triphosphate binding.Phylogenetic analysis revealed that Mvi-pgFAR with other lepidopteran pgFARs formed an independent clade.Mvi-pgFAR was specifically expressed only in the pheromone gland.Quantitative real-time polymerase chain reaction showed that the diurnal expression levels of Mvi-pgFAR in the pheromone gland were the highest at 2 h before the scotophase.After primarily con-firming Mvi-pgFAR suppression by RNAi,(E,E)-10,12-hexadecadienal(E10E1 2-16:Ald),a major sex pheromone component,was quantified by gas chromatography.When Mvi-pgFAR was successfully suppressed,E10E12-16:Ald production was reduced by up to half of that of the control,and the mating rate was subsequently decreased.Our results demonstrate that Mvi-pgFAR downregulation can suppress mating behavior by changing the relative sex pheromone component ratio,suggesting that Mvi-pgFAR can be used as a novel control target.
文献关键词：
中图分类号：
作者姓名：
Wook Hyun Cha;Dae-Weon Lee
作者机构：
Department of Biosafety,Kyungsung University,Busan,Republic of Korea;Metabolomics Research Center for Functional Materials,Kyungsung University,Busan,Republic of Korea
文献出处：
引用格式：
[1]Wook Hyun Cha;Dae-Weon Lee-.Suppression of pheromone biosynthesis and mating behavior by RNA interference of pheromone gland-specific fatty acyl reductase in Maruca vitrata)[J].中国昆虫科学（英文版）,2022(04):1135-1144
A类：
Maruca,vitrata,moths,desaturases,FARs,pgFAR,Mvi,biosynthe,lepidopteran,pgFARs,scotophase,hexadecadienal,E10E1,E10E12
B类：
Suppression,pheromone,biosynthesis,mating,behavior,by,interference,gland,fatty,acyl,In,various,enzymes,such,acid,synthases,reductases,are,involved,particular,plays,important,role,converting,func,tional,group,from,carboxylic,alcohol,during,novel,was,identified,through,transcriptome,sequencing,its,To,investigate,involvement,cloned,suppressed,RNAi,harbored,several,conserved,motifs,related,NAD,binding,glycosylation,adenosine,guanosine,triphosphate,Phylogenetic,analysis,revealed,that,other,formed,independent,clade,specifically,expressed,only,Quantitative,real,polymerase,chain,reaction,showed,diurnal,expression,levels,were,highest,before,After,primarily,firming,suppression,Ald,major,sex,component,quantified,gas,chromatography,When,successfully,production,reduced,half,control,subsequently,decreased,Our,results,demonstrate,downregulation,can,changing,relative,ratio,suggesting,used,target
AB值：
0.389311
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