典型文献
Establishment of an efficient regeneration and genetic transformation system for Malus prunifolia Borkh. 'Fupingqiuzi'
文献摘要：
Malus prunifolia Borkh. 'Fupingqiuzi' has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of 'Fupingqiuzi' progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function. However, optimal regeneration and genetic transformation systems for 'Fupingqiuzi' have not yet been developed. Here, we evaluated the factors that affect the in-vitro regeneration and transformation of 'Fupingqiuzi'. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium (MS basal salts, 30 g L?1 sucrose, 8 g L?1 agar, 5 mg L?16-benzylaminopurine (6-BA), 2 mg L?1 thidiazuron (TDZ), and 1 mg L?11-naphthlcetic acid (NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime (150 mg L?1) and kanamycin (15 mg L?1). We thus established an efficient regeneration and genetic transformation system for 'Fupingqiuzi', enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.
文献关键词：
中图分类号：
作者姓名：
LIU Yu-song;WANG Hong-ying;ZHAO Yong-juan;JIN Yi-bo;LI Chao;MA Feng-wang
作者机构：
State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China
文献出处：
引用格式：
[1]LIU Yu-song;WANG Hong-ying;ZHAO Yong-juan;JIN Yi-bo;LI Chao;MA Feng-wang-.Establishment of an efficient regeneration and genetic transformation system for Malus prunifolia Borkh. 'Fupingqiuzi')[J].农业科学学报（英文）,2022(09):2615-2627
A类：
prunifolia,Fupingqiuzi,benzylaminopurine,thidiazuron,naphthlcetic
B类：
Establishment,regeneration,genetic,transformation,Malus,Borkh,significant,ecological,economic,value,plays,key,role,germplasm,development,resistance,research,However,long,juvenile,phase,high,heterozygosity,are,barriers,identification,progeny,excellent,traits,In,vitro,techniques,Agrobacterium,mediated,systems,efficiently,produce,complete,thus,enable,studies,function,optimal,have,not,yet,been,developed,Here,evaluated,factors,that,affect,best,results,were,obtained,when,transverse,leaf,sections,used,explants,they,grown,dark,three,weeks,their,adaxial,sides,contacting,medium,basal,salts,sucrose,agar,BA,TDZ,acid,NAA,this,was,optimized,after,inoculation,tumefaciens,solution,days,delayed,cultures,screened,cefotaxime,kanamycin,We,established,enabling,rapid,production,transgenic,material,These,findings,make,contribution,apple,biology
AB值：
0.562347
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