Shoot organogenesis is critical for the short-ening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees . It enables large-scale production of plants from transformed tissues. This study evaluates the effect of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and benzylaminopurine (BAP) on the organogenesis of E. cloeziana from hypocotyls and cotyledonary leaves . In the induction stage, hypocotyls and cotyledonary leaves were established in a Murashige and Skoog (MS) culture medium supplemented with NAA or TDZ. Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage. Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89 μM BAP and 0.05 μM NAA. Cot-yledonary leaves exhibited the best in vitro regeneration. The induction of adventitious buds occurred only in calluses induced from TDZ. In the differentiation stage, 4.4 μM BAP treatment promoted an increase of adventitious bud regen-eration. Micro-cuttings from regenerated shoots were accli-matized and rooted ex vitro in mini-incubators. The results confirm the establishment of an efficient protocol for the in vitro regeneration of E. cloeziana by indirect organogene-sis, providing new insights regarding cloning of this species.

Leandro Silva de Oliveira;Gilvano Ebling Brondani;Letícia Vaz Molinari;Rafaella Zanetti Dias;Gustavo Leal Teixeira;Ant?nio Natal Gon?alves;Marcílio de Almeida

Institute of Agricultural Science,Federal University of Minas Gerais,University Campus,1000,Universitária Ave.,,Montes Claros,MG 39404-547,Brazil;Laboratory of In Vitro Culture of Forest Species,Department of Forestry Sciences,Federal University of Lavras,University Campus,Central Avenue,Lavras,MG 37200-900,Brazil;Department of Biological Sciences,"Luiz de Queiroz"College of Agriculture,University of S?o Paulo(ESALQ/USP),University Campus,11,Pádua Dias Avenue,Piracicaba,SP 13418-900,Brazil

林业研究（英文版）

[1]Leandro Silva de Oliveira;Gilvano Ebling Brondani;Letícia Vaz Molinari;Rafaella Zanetti Dias;Gustavo Leal Teixeira;Ant?nio Natal Gon?alves;Marcílio de Almeida-.Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings)[J].林业研究（英文版）,2022(05):1573-1584

cloeziana,Shoot,naphthaleneacetic,thidiazuron,benzylaminopurine,cotyledonary,Adventitious,yledonary,calluses,accli,matized,incubators,organogene

Optimal,cytokinin,auxin,balance,indirect,organogenesis,Eucalyptus,production,vitro,rooted,micro,cuttings,critical,short,ening,breeding,cycles,circumvent,barrier,cloning,mature,trees,It,enables,large,scale,plants,from,transformed,tissues,This,study,evaluates,effect,acid,NAA,TDZ,BAP,hypocotyls,leaves,In,induction,stage,were,established,Murashige,Skoog,culture,medium,supplemented,Callus,cultivated,containing,only,concentrations,differentiation,buds,multiplied,elongated,WPM,Cot,exhibited,best,regeneration,adventitious,occurred,induced,treatment,promoted,increase,Micro,regenerated,shoots,mini,results,confirm,establishment,efficient,protocol,by,providing,new,insights,regarding,this,species

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