典型文献
Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticlesOO
文献摘要：
Current gene delivery methods for maize are limited to specific genotypes and depend on time-consuming and labor-intensive tissue culture tech-niques. Here, we report a new method to transfect maize that is culture-free and genotype in-dependent. To enhance efficiency of DNA entry and maintain high pollen viability of 32％-55％, trans-fection was performed at cool temperature using pollen pretreated to open the germination aperture (40％–55％). Magnetic nanoparticles (MNPs) coated with DNA encoding either red fluorescent protein (RFP),β-glucuronidase gene (GUS), enhanced green fluorescent protein (EGFP) or bialaphos resistance (bar) was delivered into pollen grains, and female florets of maize inbred lines were pollinated. Red fluorescence was detected in 22％ transfected pollen grains, and GUS stained 55％embryos at 18 d after pollination. Green fluorescence was detected in both silk filaments and immature kernels. The T1 generation of six inbred lines showed considerable EGFP or GUS transcripts (29％–74％) quantitated by polymerase chain reaction, and 5％–16％of the T1 seedlings showed immunologically active EGFP or GUS protein. Moreover, 1.41％ of the bar trans-fected T1 plants were glufosinate resistant, and heritable bar gene was integrated into the maize genome effectively as verified by DNA hybridization. These results demonstrate that exogenous DNA could be delivered efficiently into elite maize inbred lines recalcitrant to tissue culture-mediated trans-formation and expressed normally through our genotype-independent pollen transfection system.
文献关键词：
中图分类号：
作者姓名：
Zuo-Ping Wang;Zhong-Bao Zhang;Deng-Yu Zheng;Tong-Tong Zhang;Xiang-Long Li;Chun Zhang;Rong Yu;Jian-Hua Wei;Zhong-Yi Wu
作者机构：
Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology,Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Life Sciences,Capital Normal University,Beijing 100048,China
文献出处：
引用格式：
[1]Zuo-Ping Wang;Zhong-Bao Zhang;Deng-Yu Zheng;Tong-Tong Zhang;Xiang-Long Li;Chun Zhang;Rong Yu;Jian-Hua Wei;Zhong-Yi Wu-.Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticlesOO)[J].植物学报（英文版）,2022(06):1145-1156
A类：
nanoparticlesOO,transfect,bialaphos,quantitated,immunologically
B类：
Efficient,independent,maize,transformation,using,pollen,transfected,by,coated,magnetic,Current,delivery,methods,are,limited,specific,genotypes,consuming,labor,intensive,tissue,culture,tech,niques,Here,report,new,that,free,To,efficiency,entry,maintain,high,viability,was,performed,cool,temperature,pretreated,open,germination,aperture,Magnetic,MNPs,encoding,either,fluorescent,protein,RFP,glucuronidase,GUS,enhanced,green,EGFP,resistance,bar,delivered,into,grains,female,florets,inbred,lines,were,pollinated,Red,fluorescence,detected,stained,embryos,after,pollination,Green,both,silk,filaments,immature,kernels,generation,six,showed,considerable,transcripts,polymerase,chain,reaction,seedlings,active,Moreover,plants,glufosinate,resistant,heritable,integrated,genome,effectively,verified,hybridization,These,results,demonstrate,exogenous,could,be,efficiently,elite,recalcitrant,mediated,expressed,normally,through,our,transfection,system
AB值：
0.544928
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