Drought is one of the primary abiotic stress factors affecting the yield,growth,and development of soy-beans.In extreme cases,drought can reduce yield by more than 50％.The seedling stage is an important determinant of soybean growth:the number and vigor of seedlings will affect growth and yield at har-vest.Therefore,it is important to study the drought resistance of soybean seedlings.In this study,a recombinant inbred line(RIL)population comprising 234 F6:10 lines(derived from Zhonghuang 35 × Jindou 21)and a panel of 259 soybean accessions was subjected to drought conditions to identify the effects on phenotypic traits under these conditions.Using a genetic map constructed by single nucleotide polymorphism(SNPs)markers,18 quantitative trait loci(QTL)on 7 soybean chromosomes were identified in two environments.This included 9 QTL clusters identified in the RIL population.Fifty-three QTL were identified in 19 soybean chromosomes by genome-wide association analysis(GWAS)in the panel of accessions,including 69 significant SNPs(-log10(P)≤3.97).A combination of the two populations revealed that two SNPs(-logio(P)≤3.0)fell within two of the QTL(qPH7-4 and qPH7-6)confidence intervals.We not only re-located several previously reported drought-resistance genes in soybean and other crops but also identified several non-synonymous stress-related mutation site differences between the two parents,involving Glyma.07g093000,Glyma.07g093200,Glyma.07g094100 and Glyma.07g094200.One previously unreported new gene related to drought stress,Glyma.07g094200,was found by regional association analysis.The significant SNP CHR7-17619(G/T)was within an exon of the Glyma.07g094200 gene.In the RIL population,the DSP value of the"T"allele of CHR7-17619 was significantly(P＜0.05)larger than the"G"allele in different environments.The results of our study lay the groundwork for cloning and molecular marker-assisted selection of drought-resistance genes in soybeans at the seedling stage.

Yanjun Zhang;Zhangxiong Liu;Xingrong Wang;Yue Li;Yongsheng Li;Zuowang Gou;Xingzhen Zhao;Huilong Hong;Honglei Ren;Xusheng Qi;Lijuan Qiu

Institute of Crop,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Maize Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China

作物学报（英文版）

[1]Yanjun Zhang;Zhangxiong Liu;Xingrong Wang;Yue Li;Yongsheng Li;Zuowang Gou;Xingzhen Zhao;Huilong Hong;Honglei Ren;Xusheng Qi;Lijuan Qiu-.Identification of genes for drought resistance and prediction of gene candidates in soybean seedlings based on linkage and association mapping)[J].作物学报（英文版）,2022(03):830-839

Zhonghuang,Jindou,logio,qPH7,07g093000,07g093200,07g094100,07g094200,CHR7

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