Powdery mildew of wheat is a destructive disease seriously threatening yield and quality worldwide.Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease.LS5082 is a Chinese wheat breeding line with resistance to powdery mildew.Genetic analysis,using the populations of LS5082 and three susceptible parents(Shannong 29,Shimai 22 and Huixianhong),indicated that a single dominant gene,tentatively designated PmLS5082,conferred seedling resistance to different Blumeria graminis f.sp.tritici(Bgt)isolates.Bulked segregant RNA-Seq was carried out to map PmLS5082 and to profile differentially expressed genes associated with PmLS5082.PmLS5082 was mapped to a 0.7 cM genetic interval on chromosome arm 2BL,which was aligned to a 0.7 Mb physical interval of 710.3-711.0 Mb.PmLS5082 differs from the known powdery mildew(Pm)resistance genes on chromosome arm 2BL based on their origin,chromosome positions and/or resistance spectrum,sug-gesting PmLS5082 is most likely a new Pm gene/allele.Through clusters of orthologous groups and kyoto encyclopedia of genes and genomes analyses,differentially expressed genes(DEGs)associated with PmLS5082 were profiled.Six DEGs in the PmLS5082 interval were confirmed to be associated with PmLS5082 via qPCR analysis,using an additional set of wheat samples and time-course analysis post-inoculation with Bgt isolate E09.Ten closely linked markers,including two kompetitive allele-specific PCR markers,were confirmed to be suitable for marker-assisted selection of PmLS5082 in different genetic backgrounds,thus can be used to detect PmLS5082 and pyramid it with other genes in breeding programs.

Liru Wu;Tong Zhu;Huagang He;Xinyou Cao;Haosheng Li;Hongxing Xu;Mengshu Jia;Lipei Zhang;Jiancheng Song;Ghader Mirzaghaderi;Cheng Liu;Pengtao Ma

School of Life Sciences,Yantai University,Yantai 264005,Shandong,China;School of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China;Crop Research Institute,Shandong Academy of Agriculture Sciences,Jinan 250100,Shandong,China;State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,Henan,China;Yantai Jien Biological Science&Technology Ltd.,Yantai 265100,Shandong,China;Department of Agronomy and Plant Breeding,Faculty of Agriculture,University of Kurdistan,P.O.Box 416,Sanandaj,Iran

作物学报（英文版）

[1]Liru Wu;Tong Zhu;Huagang He;Xinyou Cao;Haosheng Li;Hongxing Xu;Mengshu Jia;Lipei Zhang;Jiancheng Song;Ghader Mirzaghaderi;Cheng Liu;Pengtao Ma-.Genetic dissection of the powdery mildew resistance in wheat breeding line LS5082 using BSR-Seq)[J].作物学报（英文版）,2022(04):1120-1130

LS5082,Shannong,Shimai,Huixianhong,PmLS5082,2BL,E09

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