Deoxynivalenol(DON)is considered to be the most harmful mycotoxin that affects the intestinal health of animals and humans.Phenethyl isothiocyanate(PEITC)in feedstuff is an anti-nutritional factor and impairs nutrient digestion and absorption in the animal intestinal.In the current study,we aimed to explore the effects of PEITC on DON-induced apoptosis,intestinal tight junction disorder,and its po-tential molecular mechanism in the porcine jejunum epithelial cell line(IPEC-J2).Our results indicated that PEITC treatment markedly alleviated DON-induced cytotoxicity,decreasing the apoptotic cell per-centage and pro-apoptotic mRNA/protein levels,and increasing zonula occludens-1(ZO-1),occludin and claudin-1 mRNA/protein expression.Meanwhile,PEITC treatment ameliorated DON-induced an increase of the inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)mRNA levels and intracellular reactive oxygen species(ROS)level,and a decrease of glutathione peroxidase 1(GPx1),superoxide dis-mutase 2(SOD2),catalase(CAT)and heme oxygenase 1(HO-1)mRNA levels.Additionally,PEITC treat-ment significantly down-regulated autophagy-related protein 5(ATG5),beclin-1 and microtubule-associated protein 1 light chain 3B(LC3-Ⅱ)mRNA/protein levels,decreased the number of green fluo-rescent protein-microtubule-associated protein 1 light-chain 3(GFP-LC3)puncta and phosphatidylino-sitol 3 kinase(PI3K)protein expression,and up-regulated phospho-protein kinase B(p-Akt)and phospho-mammalian target of rapamycin(p-mTOR)protein expression against DON.However,the activation of autophagy by rapamycin,an autophagy agonist,abolished the protective effects of PEITC against DON-induced cytotoxicity,apoptosis and intestinal tight junction disorder.Collectively,PEITC could confer protection against DON-induced porcine intestinal epithelial cell injury by suppressing ROS-mediated autophagy.

Shuiping Liu;Xinru Mao;Lei Ge;Lili Hou;Guannan Le;Fang Gan;Lixin Wen;Kehe Huang

College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,210095,China;Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls,Nanjing Agricultural University,Nanjing,210095,China;MOE Joint International Research Laboratory of Animal Health and Food Safety,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,210095,China;College of Veterinary Medicine,Hunan Agricultural University,Changsha,410128,China

动物营养（英文）

[1]Shuiping Liu;Xinru Mao;Lei Ge;Lili Hou;Guannan Le;Fang Gan;Lixin Wen;Kehe Huang-.Phenethyl isothiocyanate as an anti-nutritional factor attenuates deoxynivalenol-induced IPEC-J2 cell injury through inhibiting ROS-mediated autophagy)[J].动物营养（英文）,2022(01):300-309

Phenethyl,PEITC,phosphatidylino,sitol

isothiocyanate,anti,nutritional,attenuates,deoxynivalenol,induced,IPEC,J2,injury,through,inhibiting,ROS,mediated,autophagy,Deoxynivalenol,DON,considered,most,harmful,mycotoxin,that,affects,intestinal,health,animals,humans,feedstuff,impairs,nutrient,digestion,absorption,In,current,study,aimed,explore,effects,apoptosis,tight,junction,disorder,its,tential,molecular,mechanism,porcine,jejunum,epithelial,line,Our,results,indicated,treatment,markedly,alleviated,cytotoxicity,decreasing,apoptotic,centage,protein,levels,increasing,zonula,occludens,ZO,occludin,claudin,expression,Meanwhile,ameliorated,increase,inducible,nitric,synthase,iNOS,cyclooxygenase,COX,intracellular,reactive,species,glutathione,peroxidase,GPx1,superoxide,mutase,SOD2,catalase,CAT,heme,HO,Additionally,significantly,down,regulated,related,ATG5,beclin,microtubule,associated,light,chain,3B,LC3,decreased,number,green,fluo,rescent,GFP,puncta,kinase,PI3K,phospho,Akt,mammalian,target,rapamycin,mTOR,against,However,activation,by,agonist,abolished,protective,Collectively,could,confer,protection,suppressing

0.52656