典型文献
Amygdalin attenuates PM2.5-induced human umbilical vein endothelial cell injury via the TLR4/NF-KB and Bcl-2/Bax signaling pathways
文献摘要：
Mounting evidence supports that long-term exposure to fine particle pollutants(PM2.5)is closely implicated in cardiovascular diseases,especially atherosclerosis.Amygdalin is reported to attenuate external stimuli-induced cardiovascular diseases.However,the underlying mechanisms are still not understood.In this study,we aim to explore the protective effects of amygdalin on PM2.5-induced human umbilical vein endothelial cell(HUVEC)injury and unravel the specific mechanisms by MTT,DCFH-DA,biochemical,immunofluorescence,ELISA,RT-qPCR,flow cytometry,TUNEL and western blot analysis.The results reveal that amygdalin reverses PM2.5-induced cytotoxicity and attenuates intracellular ROS production.Moreover,amygdalin increases the levels of SOD and GSH and alleviates the MDA content.Additionally,amygdalin causes a decline of IL-6,IL-1β,TNF-α and COX-2 levels.Moreover,amygdalin inhibits NF-κB p50 and TLR4 protein expressions and NF-κB p65 nuclear translocation.Concomitantly,a decline of phospho-NF-KB p65/NF-κB p65 and phospho-IKB-α/IKB-α is detected.Meanwhile,amygdalin pretreatment reduces HUVEC apoptosis.In addition,amygdalin triggers an upregulation of Bcl-2 and a downregulation of Bax after stimulation with PM2.5.Collectively,these results suggest that amygdalin suppresses PM2.5-induced HUVEC injury by regulating the TLR4/NF-κB and Bcl-2/Bax signaling pathways,indicating that amygdalin may be a novel target for atherosclerosis treatments.
文献关键词：
中图分类号：
作者姓名：
Bixu Wang;Tong Sun;Ling Sun;Lan Li;Haitong Wan;Zhishan Ding;Xiaoqing Ye
作者机构：
School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University,Hangzhou 310053,China;School of Life Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China
文献出处：
引用格式：
[1]Bixu Wang;Tong Sun;Ling Sun;Lan Li;Haitong Wan;Zhishan Ding;Xiaoqing Ye-.Amygdalin attenuates PM2.5-induced human umbilical vein endothelial cell injury via the TLR4/NF-KB and Bcl-2/Bax signaling pathways)[J].生物化学与生物物理学报（英文版）,2022(10):1476-1485
A类：
Amygdalin,Concomitantly
B类：
attenuates,PM2,induced,human,umbilical,vein,endothelial,injury,TLR4,Bcl,Bax,signaling,pathways,Mounting,evidence,supports,that,long,term,exposure,fine,particle,pollutants,closely,implicated,cardiovascular,diseases,especially,atherosclerosis,reported,external,stimuli,However,underlying,mechanisms,are,still,not,understood,In,this,study,aim,explore,protective,effects,amygdalin,HUVEC,unravel,specific,by,MTT,DCFH,biochemical,immunofluorescence,qPCR,flow,cytometry,TUNEL,western,blot,analysis,results,reveal,reverses,cytotoxicity,intracellular,ROS,production,Moreover,increases,levels,GSH,alleviates,content,Additionally,causes,decline,COX,inhibits,p50,protein,expressions,p65,nuclear,translocation,phospho,IKB,detected,Meanwhile,pretreatment,reduces,apoptosis,addition,triggers,upregulation,downregulation,after,stimulation,Collectively,these,suggest,suppresses,regulating,indicating,may,be,novel,target,treatments
AB值：
0.547758
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