典型文献
Ginsenoside Rb1 improves brain,lung,and intestinal barrier dam-age in middle cerebral artery occlusion/reperfusion(MCAO/R)mice via the PPARγ signaling pathway
文献摘要：
Ischemic stroke causes brain inflammation and multi-organ injury,which is closely associated with the peroxisome proliferator-activated receptor-gamma(PPARγ)signaling pathway.Recent studies have indicated that ginsenoside Rb1(GRb1)can protect the integrity of the blood-brain barrier after stroke.In the current study,a mouse model of middle cerebral artery occlusion/reperfusion(MCAO/R)was established to determine whether GRb1 can ameliorate brain/lung/intestinal barrier damage via the PPARγ signaling pathway.Staining(2,3,5-triphenyltetrazolium chloride,hematoxylin,and eosin)and Doppler ultrasonography were employed to detect pathological changes.Endothelial breakdown was investigated with the leakage of Evans Blue dye and the ex-pression of TJs(tight junctions)and AJs(adherent junctions).Western blot and immunofluorescence were used to determine the levels of cell junction proteins,PPARγ and NF-κB.Results showed that GRb1 significantly mitigated multi-organ injury and increased the expression of cerebral microvascular,pulmonary vascular,and intestinal epithelial connexins.In brain,lung,and intestinal tissues,GRb1 activated PPARγ,decreased the levels of phospho-NF-κB p65,and inhibited the production of proinflammatory cytokines,thereby maintaining barrier permeability.However,co-treatment with GRb1 and the PPARγ antagonist GW9662 reversed the barrier-protective effect of GRb1.These findings indicated that GRb1 can improve stroke-induced brain/lung/intestinal barrier damage via the PPARγ pathway.
文献关键词：
中图分类号：
作者姓名：
SU Lin-Jie;REN Yu-Chuan;CHEN Zhuo;MA Hui-Fen;ZHENG Fan;LI Fang;ZHANG Yuan-Yuan;GONG Shuai-Shuai;KOU Jun-Ping
作者机构：
Jiangsu Key Laboratory of TCM Evaluation and Translational Research,Department of Pharmacology of Chinese Materia Medica,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China
文献出处：
引用格式：
[1]SU Lin-Jie;REN Yu-Chuan;CHEN Zhuo;MA Hui-Fen;ZHENG Fan;LI Fang;ZHANG Yuan-Yuan;GONG Shuai-Shuai;KOU Jun-Ping-.Ginsenoside Rb1 improves brain,lung,and intestinal barrier dam-age in middle cerebral artery occlusion/reperfusion(MCAO/R)mice via the PPARγ signaling pathway)[J].中国天然药物,2022(08):561-571
A类：
Staining,AJs,connexins
B类：
Ginsenoside,improves,brain,lung,intestinal,barrier,middle,cerebral,artery,occlusion,reperfusion,MCAO,mice,via,PPAR,signaling,pathway,Ischemic,stroke,causes,inflammation,multi,organ,injury,which,closely,associated,peroxisome,proliferator,activated,receptor,gamma,Recent,studies,have,indicated,that,ginsenoside,GRb1,integrity,blood,after,In,current,study,mouse,model,was,established,determine,whether,ameliorate,damage,triphenyltetrazolium,chloride,hematoxylin,eosin,Doppler,ultrasonography,were,employed,detect,pathological,changes,Endothelial,breakdown,investigated,leakage,Evans,Blue,dye,TJs,tight,junctions,adherent,blot,immunofluorescence,used,levels,cell,proteins,Results,showed,significantly,mitigated,increased,expression,microvascular,pulmonary,epithelial,tissues,decreased,phospho,p65,inhibited,production,proinflammatory,cytokines,thereby,maintaining,permeability,However,treatment,antagonist,GW9662,reversed,protective,effect,These,findings,induced
AB值：
0.53346
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