Objective:To investigate the protective effects of modified Linggui Zhugan Decoction(MLZD),a traditional Chinese medicine formula,on obese type 2 diabetes mellitus (T2DM) rats.Methods:Fifty Sprague-Dawley rats were randomly divided into 5 groups by a random number table,including normal,obese T2DM (ob-T2DM),MLZD low-dose[MLDZ-L,4.625 g/(kg·d)],MLZD middle-dose[MLD-M,9.25 g/(kg·d)]and MLZD high-dose[MLD-H,18.5 g/(kg·d)]groups,10 rats in each group.After 4-week intervention,blood samples and liver,pancreas,muscle tissues were collected to assess the insulin resistance(IR),blood lipid,adipokines and inflammation cytokines.The alteration of phosphatidylinositol 3 kinase (PI3K)-protein kinase B (PKB or Akt)/the mammalian target of rapamycin (mTOR)-ribosome protein subunit 6 kinase 1(S6K1)/AMP-activated protein kinase (AMPK)-peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 o) pathways were also studied.Results:MLZD dose-dependently reduced fasting blood glucose,fasting insulin,homeostasis model of assessment for IR index and increased insulin sensitive index compared with ob-T2DM rats (P＜0.05).Similarly,total cholesterol,triglyceride,low-density lipoprotein cholesterol and free fatty acids were also decreased compared with ob-T2DM rats after 4-week treatment (P＜0.05 or P＜0.01).Improvements in adipokines and inflammatory cytokines were observed with a raised level of adiponectin and a reduced level of leptin,resistin,tumor necrosis factor-α and interleukin-6 (P＜0.05 or P＜0.01).MLZD regulated the PI3K-Akt/mTOR-S6K1/AMPK-PGC-1α pathways and restored the tissue structure of liver and pancreas(P＜0.05 or P＜0.01).Conclusions;MLZD ameliorated glycolipid metabolism and inflammation,which may be attributed to the regulation of PI3K-Akt/mTOR-S6K1/AMPK-PGC-1α pathways.

SUN Jia-pan;SHI Lin;WANG Fang;QIN Jian;KE Bin

Department of Traditional Chinese Medicine,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080 ,China;Department of Traditional Chinese Medicine,Zhujiang Hospital of Southern Medical University,Guangzhou 510280 ,China;Department of Oncology,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080 ,China;Department of Traditional Chinese Medicine,the Seventh Affiliated Hospital,Sun Yat-sen University,Shenzhen 518107 ,Guangdong Province,China;Department of VIP Ward,Sun Yat-sen University Cancer Center,Guangzhou 510060 ,China

中国结合医学杂志（英文版）

[1]SUN Jia-pan;SHI Lin;WANG Fang;QIN Jian;KE Bin-.Modified Linggui Zhugan Decoction Ameliorates Glycolipid Metabolism and Inflammation via PI3K-Akt/mTOR-S6K1/AMPK-PGC-1α Signaling Pathways in Obese Type 2 Diabetic Rats)[J].中国结合医学杂志（英文版）,2022(01):52-59

Linggui,Zhugan,Glycolipid,MLZD,MLDZ,adipokines

Modified,Decoction,Ameliorates,Metabolism,Inflammation,via,PI3K,Akt,mTOR,S6K1,AMPK,PGC,Signaling,Pathways,Obese,Type,Diabetic,Rats,Objective,To,investigate,protective,effects,modified,traditional,Chinese,medicine,formula,obese,type,diabetes,mellitus,T2DM,rats,Methods,Fifty,Sprague,Dawley,were,randomly,divided,into,groups,by,number,table,including,normal,low,dose,middle,high,each,After,week,intervention,blood,samples,liver,pancreas,muscle,tissues,collected,insulin,resistance,inflammation,cytokines,alteration,phosphatidylinositol,kinase,PKB,mammalian,target,rapamycin,ribosome,subunit,activated,peroxisome,proliferator,receptor,gamma,coactivator,alpha,pathways,also,studied,Results,dependently,reduced,fasting,glucose,homeostasis,model,assessment,increased,sensitive,compared,Similarly,total,cholesterol,triglyceride,density,lipoprotein,free,fatty,acids,decreased,after,treatment,Improvements,inflammatory,observed,raised,level,adiponectin,leptin,resistin,tumor,necrosis,interleukin,regulated,restored,structure,Conclusions,ameliorated,glycolipid,metabolism,which,may,attributed,regulation

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