Impaired immunomodulatory capacity and oxidative stress are the key factors limiting the effectiveness of mesen-chymal stem cell transplantation therapy.The present study was aimed to investigate the effects of jujuboside A(JuA)on the protect-ive effect and immunomodulatory capacity of human umbilical cord mesenchymal stem cells(hUC-MSCs).Hydrogen peroxide was used to establish an oxidative damage model of hUC-MSCs,while PBMCs isolated from rats were used to evaluate the effect of JuA pre-treatment on the immunomodulatory capacity of hUC-MSCs.Furthermore,Hoechst 33258 staining,lactate dehydrogenase test,measurement of malondialdehyde,Western blot,high-performance liquid chromatography;and flow cytometry were performed.Our results indicated that JuA(25 μmol·L-1)promoted the proliferation of hUC-MSCs,but did not affect the differentiating capability of these cells.JuA pre-treatment inhibited apoptosis,prevented oxidative damage,and up-regulated the protein expression of nuclear factor-erythroid factor 2-related factor 2 and heme oxygenase 1 in hUC-MSCs in which oxidative stress was induced with H2O2.In ad-dition,JuA pre-treatment enhanced the inhibitory effect of hUC-MSCs against abnormally activated PBMCs,which was related to stimulation of the expression and activity of indoleamine 2,3-dioxygenase.In conclusion,our results demonstrate that JuA pre-treat-ment can enhance the survival and immunomodulatory ability through pathways related to oxidative stress,providing a new option for the improvement of hUC-MSCs in the clinical setting.

School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Department of Neurology,Dalian Municipal Central Hospital Affiliated to Dalian Medical University,Dalian 116033,China

[1]CHEN Ji-Cong;XIAO Hong-He;ZHANG Qiang;KONG Liang;WANG Tian-Min;TIAN Yu;ZHAO Yu-Meng;LI He;TIAN Jin-Ming;WANG Cui;YANG Jing-Xian-.Jujuboside A inhibits oxidative stress damage and enhances immun-omodulatory capacity of human umbilical cord mesenchymal stem cells through up-regulating IDO expression)[J].中国天然药物,2022(07):494-505

Jujuboside,immun,omodulatory,jujuboside,JuA

inhibits,oxidative,stress,damage,enhances,capacity,human,umbilical,cord,mesenchymal,stem,cells,through,up,regulating,IDO,expression,Impaired,immunomodulatory,are,key,factors,limiting,effectiveness,transplantation,therapy,present,study,was,aimed,investigate,effects,protect,hUC,MSCs,Hydrogen,peroxide,used,establish,model,while,PBMCs,isolated,from,rats,were,evaluate,treatment,Furthermore,Hoechst,staining,lactate,dehydrogenase,test,measurement,malondialdehyde,blot,high,performance,liquid,chromatography,flow,cytometry,performed,Our,results,indicated,that,promoted,proliferation,but,did,not,affect,differentiating,capability,these,inhibited,apoptosis,prevented,regulated,protein,nuclear,erythroid,related,heme,which,induced,H2O2,In,ad,dition,enhanced,inhibitory,against,abnormally,activated,stimulation,activity,indoleamine,dioxygenase,conclusion,our,demonstrate,can,survival,pathways,providing,new,option,improvement,clinical,setting

0.487998