Objective:To evaluate the therapeutic effects of acupoint autohemotherapy(A-AHT)on 1-chloro-2,4-dinitrobenzene(DNCB)-induced atopic dermatitis(AD)in mice focusing on regulating T helper 1/T helper 2(Th1/Th2)immune responses.Methods:Thirty BALB/c mice were divided into 5 groups by a random number table,including normal control(NC),AD model(AD),A-AHT,sham A-AHT(sA-AHT),and acupoint injection of normal saline(A-NS)groups,6 mice per group.Mice were challenged by DNCB for the establishment of experimental AD model.On the 8th day,except for the NC and AD groups,the mice in the other groups received management once every other day for a total of 28 days.For the A-AHT and sA-AHT groups,0.05 mL of autologous whole blood(AWB)was injected into bilateral Zusanli(ST 36)and Quchi(LI 11)and sham-acupoints(5 mm lateral to ST 36 and LI 11),respectively.The A-NS group was administrated with 0.05 mL of normal saline by acupoint injection into ST 36 and LI 11.Dermatitis severity for dorsal skin of mice was determined using the Severity Scoring of Atopic Dermatitis(SCORAD)every week.The total immunoglobulin E(IgE),interleukin-4(IL-4),and interferon-gamma(IFN-γ)cytokine levels in serum were examined by enzyme-linked immunosorbent assay(ELISA).Spleen Th1/Th2 expression were analyzed via flow cytometry and immunohistochemical assay was used to detect T-box expressed in T cell(T-bet)and GATA-binding protein 3(GATA3)expressions in skin lesions of mice.Results:Compared with the AD group,both A-AHT and sA-AHT reduced the SCORAD index and serum IgE level(P＜0.05 or P＜0.01);A-AHT,sA-AHT and A-NS down-regulated serum IL-4 level and up-regulated IFN-γ/IL-4 ratio(P＜0.05 or P＜0.01);A-AHT regulated the Th1/Th2 shift specifically and increased the related transcription factors such as T-bet expression and T-bet/GATA3 ratio(P＜0.05).Conclusion:A-AHT showed significant effectiveness on the AD model mice,through regulating Th1/Th2 immune responses.

ZENG Zhi-wen;HUANG Jin-quan;CHEN Yong;YU Xiao;ZHU Wei;ZHANG Dong-shu

School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510515,China;Department of Traditional Chinese Medicine,Affiliated Dongguan People's Hospital,Southern Medical University,Dongguan,Guangdong Province,523058,China;Department of Rheumatology and Immunology,Shenzhen People's Hospital,The Second Clinical Medical College of Jinan University,Shenzhen,Guangdong Province,518020,China;School of Basic Medical Sciences,Southern Medical University,Guangzhou,510515,China;Guangzhou Center for Disease Control and Prevention,Guangzhou,510515,China

中国结合医学杂志（英文版）

[1]ZENG Zhi-wen;HUANG Jin-quan;CHEN Yong;YU Xiao;ZHU Wei;ZHANG Dong-shu-.Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice)[J].中国结合医学杂志（英文版）,2022(07):612-619

Autohemotherapy,autohemotherapy,dinitrobenzene,AWB,Quchi

Acupoint,Attenuates,Atopic,Dermatitis,Lesions,by,Regulating,Th1,Th2,Balance,DNCB,Induced,BALB,Mice,Objective,To,evaluate,therapeutic,effects,AHT,chloro,induced,atopic,dermatitis,mice,focusing,regulating,helper,immune,responses,Methods,Thirty,were,divided,into,groups,random,number,table,including,normal,control,model,sham,sA,injection,saline,NS,challenged,establishment,experimental,On,8th,except,received,management,once,every,total,days,For,autologous,whole,blood,was,injected,bilateral,Zusanli,ST,acupoints,respectively,administrated,severity,dorsal,skin,determined,Severity,Scoring,SCORAD,week,immunoglobulin,IgE,interleukin,interferon,gamma,IFN,cytokine,levels,serum,examined,enzyme,linked,immunosorbent,assay,Spleen,analyzed,via,flow,cytometry,immunohistochemical,used,detect,box,expressed,cell,bet,binding,protein,GATA3,expressions,lesions,Results,Compared,both,reduced,down,regulated,ratio,shift,specifically,increased,related,transcription,factors,such,Conclusion,showed,significant,effectiveness,through

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