典型文献
Validamycin treatment significantly inhibits the glycometabolism and chitin synthesis in the common cutworm,Spodoptera litura
文献摘要：
Validamycin,as a broadly applied antibiotic,has been used to control rice sheath blight disease.Furthermore,validamycin was considered as an insecticide to con-trol agricultural pests.Insight into the mechanism of validamycin's action on insects can provide molecular targets for the control of agricultural pests.In this study,a toxicological test analysis revealed that Spodoptera litura larval growth and development was signifi-cantly inhibited and the pupation rate was significantly reduced with the increase of the concentration of validamycin.According to the NMR-based metabolomic analysis,a total of 15 metabolites involved in glycolysis and tricarboxylic acid cycle(TCA)pathways were identified.Additionally,trehalase activities,glucose and chitin contents were significantly downregulated,but the trehalose content was upregulated after exposure to validamycin.Reverse transcription quantitative PCR analysis revealed that the expression level of genes involved in glycolysis,TCA and chitin synthesis were upregulated after treating with val-idamycin.Further chitin staining also confirmed that chitin content was downregulated at 12 h after validamycin treatment.Our results indicated that validamycin worked via two different molecular mechanisms,one through inhibiting glycometabolism and the other by inhibiting chitin synthesis in S.litura.The information lays a theoretical foundation for further control of S.litura.
文献关键词：
中图分类号：
作者姓名：
Hai-Zhong Yu;Qin Zhang;Zhan-Jun Lu;Ming-Jie Deng
作者机构：
College of Life Sciences,Gannan Normal University,Ganzhou,Jiangxi Province,China;National Navel Orange Engineering Research Center,Ganzhou,Jiangxi Province,China;Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,Zhejiang Province,China
文献出处：
引用格式：
[1]Hai-Zhong Yu;Qin Zhang;Zhan-Jun Lu;Ming-Jie Deng-.Validamycin treatment significantly inhibits the glycometabolism and chitin synthesis in the common cutworm,Spodoptera litura)[J].中国昆虫科学（英文版）,2022(03):840-854
A类：
Validamycin,glycometabolism,cutworm,validamycin,pupation,idamycin
B类：
treatment,significantly,inhibits,chitin,synthesis,common,Spodoptera,litura,broadly,applied,antibiotic,has,been,used,control,rice,sheath,blight,disease,Furthermore,was,considered,insecticide,agricultural,pests,Insight,into,action,insects,provide,molecular,targets,this,study,toxicological,test,analysis,revealed,that,larval,growth,development,inhibited,rate,reduced,increase,concentration,According,NMR,metabolomic,total,metabolites,involved,glycolysis,tricarboxylic,acid,cycle,TCA,pathways,were,identified,Additionally,trehalase,activities,glucose,contents,downregulated,but,trehalose,upregulated,after,exposure,Reverse,transcription,quantitative,expression,level,genes,treating,staining,also,confirmed,Our,results,indicated,worked,via,different,mechanisms,one,through,inhibiting,other,by,information,lays,theoretical,foundation,further
AB值：
0.494729
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